Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit N-methyltransferase
Abstract
The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) .epsilon.N-methyltransferase (protein methylase III or Rubisco LSMT) is disclosed. This enzyme catalyzes methylation of the .epsilon.-amine of lysine-14 in the large subunit of Rubisco. In addition, a full-length cDNA clone for Rubisco LSMT is disclosed. Transgenic plants and methods of producing same which (1) have the Rubisco LSMT gene inserted into the DNA, and (2) have the Rubisco LSMT gene product or the action of the gene product deleted from the DNA are also provided. Further, methods of using the gene to selectively deliver desired agents to a plant are also disclosed.
- Inventors:
-
- Lexington, KY
- Publication Date:
- Research Org.:
- University Of Kentucky (Lexington, KY)
- OSTI Identifier:
- 871399
- Patent Number(s):
- US 5723752
- Assignee:
- University Of Kentucky (Lexington, KY)
- DOE Contract Number:
- FG05-92ER20075
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- cloning; developmental; expression; pea; ribulose-1; 5-bisphosphate; carboxylase; oxygenase; subunit; n-methyltransferase; sequence; rubisco; epsilon; protein; methylase; iii; lsmt; disclosed; enzyme; catalyzes; methylation; -amine; lysine-14; addition; full-length; cdna; clone; transgenic; plants; methods; producing; inserted; dna; product; action; deleted; provided; selectively; deliver; desired; agents; plant; cdna clone; rubisco lsmt; transgenic plants; protein methylase; methylase iii; full-length cdna; pea ribulose-1; enzyme catalyzes; transgenic plant; developmental expression; /800/435/999/
Citation Formats
Houtz, Robert L. Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit N-methyltransferase. United States: N. p., 1998.
Web.
Houtz, Robert L. Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit N-methyltransferase. United States.
Houtz, Robert L. Thu .
"Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit N-methyltransferase". United States. https://www.osti.gov/servlets/purl/871399.
@article{osti_871399,
title = {Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit N-methyltransferase},
author = {Houtz, Robert L},
abstractNote = {The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) .epsilon.N-methyltransferase (protein methylase III or Rubisco LSMT) is disclosed. This enzyme catalyzes methylation of the .epsilon.-amine of lysine-14 in the large subunit of Rubisco. In addition, a full-length cDNA clone for Rubisco LSMT is disclosed. Transgenic plants and methods of producing same which (1) have the Rubisco LSMT gene inserted into the DNA, and (2) have the Rubisco LSMT gene product or the action of the gene product deleted from the DNA are also provided. Further, methods of using the gene to selectively deliver desired agents to a plant are also disclosed.},
doi = {},
url = {https://www.osti.gov/biblio/871399},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {1}
}
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