Cellulase-containing cell-free fermentate produced from microorganism ATCC 55702
Abstract
Bacteria which produce large amounts of cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.
- Inventors:
-
- Lenoir City, TN
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- OSTI Identifier:
- 871281
- Patent Number(s):
- US 5698429
- Application Number:
- 08/729819
- Assignee:
- Lockheed Martin Energy Systems, Inc. (Oak Ridge, TN)
- DOE Contract Number:
- AC05-84OR21400
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- cellulase-containing; cell-free; fermentate; produced; microorganism; atcc; 55702; bacteria; produce; amounts; identified; original; bacterium; 55703; genetically; altered; nitrosoguanidine; mnng; treatment; enhanced; cellulase; producing; replicate; plating; improved; characteristics; qualities; degradation; cellulosic; waste; materials; fuel; production; food; processing; textile; industrial; applications; bacterial; host; genetic; manipulations; recombinant; dna; techniques; destroy; standard; mutagenesis; cellulase producing; cellulase-containing cell-free; industrial applications; improved characteristics; original bacterium; bacterial host; enhanced cellulase; replicate plating; waste material; waste materials; recombinant dna; genetically altered; cell-free fermentate; genetic manipulations; standard mutagenesis; microorganism atcc; mutagenesis techniques; fuel production; textile processing; fermentate produced; dna techniques; destroy genetic; improved bacterial; improved bacteria; food processing; cellulosic waste; producing bacterium; fuel product; /435/
Citation Formats
Dees, H Craig. Cellulase-containing cell-free fermentate produced from microorganism ATCC 55702. United States: N. p., 1997.
Web.
Dees, H Craig. Cellulase-containing cell-free fermentate produced from microorganism ATCC 55702. United States.
Dees, H Craig. 1997.
"Cellulase-containing cell-free fermentate produced from microorganism ATCC 55702". United States. https://www.osti.gov/servlets/purl/871281.
@article{osti_871281,
title = {Cellulase-containing cell-free fermentate produced from microorganism ATCC 55702},
author = {Dees, H Craig},
abstractNote = {Bacteria which produce large amounts of cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.},
doi = {},
url = {https://www.osti.gov/biblio/871281},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Dec 16 00:00:00 EST 1997},
month = {Tue Dec 16 00:00:00 EST 1997}
}
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