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Title: DNA complexes with dyes designed for energy transfer as fluorescent markers

Patent ·
OSTI ID:871043
 [1];  [2]
  1. Orinda, CA
  2. Albany, CA
+ Show Author Affiliations

Heteromultimeric fluorophores are provided for binding to DNA, which allow for the detection of DNA in electrical separations and preparation of probes having high-fluorescent efficiencies and large Stokes shifts. In addition, by appropriate choice of fluorescent molecules, one can use a single narrow wavelength band excitation light source, while obtaining fluorescent emissions having sufficient separation to be readily discriminated.

Research Organization:
Univ. of California (United States)
DOE Contract Number:
FG03-91ER61125
Assignee:
Regents of University of California (Oakland, CA)
Patent Number(s):
US 5646264
OSTI ID:
871043
Country of Publication:
United States
Language:
English

References (9)

The Interaction of Intercalating Drugs with Nucleic Acids journal June 1981
Design of Bifunctional Nucleic Acid Ligands book January 1990
DNA bifunctional intercalators. 1. Synthesis and conformational properties of an ethidium homodimer and of an acridine ethidium heterodimer journal November 1978
Ethidium dimer: A new reagent for the fluorimetric determination of nucleic acids journal April 1979
DNA bifunctional intercalators. 2. Fluorescence properties and DNA binding interaction of an ethidium homodimer and an acridine ethidium heterodimer. Appendix: Numerical solution of McGhee and von Hippel equations for competing ligands journal November 1978
Dynamic structure of DNA complexes. Fluorometric measurement of hydrogen-deuterium exchange kinetics of DNA-bound ethidium dimer and acridine-ethidium dimer journal June 1983
High-sensitivity two-color detection of double-stranded DNA with a confocal fluorescence gel scanner using ethidium homodimer and thiazole orange journal January 1991
Effect of B-Z transition and nucleic add structure on the conformational dynamics of bound ethidium dimer measured by hydrogen deuterium exchange kinetics journal January 1985
Stable fluorescent complexes of double-stranded DNA with bis-intercalating asymmetric cyanine dyes: properties and applications journal January 1992

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dna
complexes
dyes
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energy
transfer
fluorescent
markers
heteromultimeric
fluorophores
provided
binding
allow
detection
electrical
separations
preparation
probes
high-fluorescent
efficiencies
stokes
shifts
addition
appropriate
choice
molecules
single
narrow
wavelength
band
excitation
light
source
obtaining
emissions
sufficient
separation
readily
discriminated
stokes shifts
dyes designed
fluorescent molecules
single narrow
energy transfer
light source
excitation light
fluorescent emissions
wavelength band
narrow wavelength
readily discriminated
obtaining fluorescent
sufficient separation
electrical separations
dna complexes
high-fluorescent efficiencies
heteromultimeric fluorophores
fluorescent markers
band excitation
appropriate choice
fluorescent emission
stokes shift
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