System for sensing droplet formation time delay in a flow cytometer

Patent · Wed Jan 01 00:00:00 EST 1997

OSTI ID:871026

Van den Engh, Ger ^[1]; Esposito, Richard J ^[1]

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1. Seattle, WA

A droplet flow cytometer system which includes a system to optimize the droplet formation time delay based on conditions actually experienced includes an automatic droplet sampler which rapidly moves a plurality of containers stepwise through the droplet stream while simultaneously adjusting the droplet time delay. Through the system sampling of an actual substance to be processed can be used to minimize the effect of the substances variations or the determination of which time delay is optimal. Analysis such as cell counting and the like may be conducted manually or automatically and input to a time delay adjustment which may then act with analysis equipment to revise the time delay estimate actually applied during processing. The automatic sampler can be controlled through a microprocessor and appropriate programming to bracket an initial droplet formation time delay estimate. When maximization counts through volume, weight, or other types of analysis exists in the containers, the increment may then be reduced for a more accurate ultimate setting. This may be accomplished while actually processing the sample without interruption.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
• DOE Contract Number: FG06-93ER61662
• Assignee: University of Washington (Seattle, WA)
• Patent Number(s): US 5643796
• OSTI ID: 871026
• Country of Publication: United States
• Language: English

Related Subjects

sensing
droplet
formation
time
delay
flow
cytometer
optimize
based
conditions
experienced
automatic
sampler
rapidly
moves
plurality
containers
stepwise
stream
simultaneously
adjusting
sampling
substance
processed
minimize
effect
substances
variations
determination
optimal
analysis
cell
counting
conducted
manually
automatically
input
adjustment
equipment
revise
estimate
applied
processing
controlled
microprocessor
appropriate
programming
bracket
initial
maximization
counts
volume
weight
types
exists
increment
reduced
accurate
ultimate
setting
accomplished
sample
interruption
droplet formation
time delay
flow cytometer
droplet stream
formation time
delay based
/436/209/250/361/422/435/