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Title: Method and apparatus for detection of fluorescently labeled materials

Patent ·
OSTI ID:870962
 [1];  [2]
  1. Mt. View, CA
  2. Los Altos, CA
+ Show Author Affiliations

Fluorescently marked targets bind to a substrate 230 synthesized with polymer sequences at known locations. The targets are detected by exposing selected regions of the substrate 230 to light from a light source 100 and detecting the photons from the light fluoresced therefrom, and repeating the steps of exposure and detection until the substrate 230 is completely examined. The resulting data can be used to determine binding affinity of the targets to specific polymer sequences.

Research Organization:
Affymax Research Institute
DOE Contract Number:
FG03-92ER81275
Assignee:
Affymetrix, Inc. (Santa Clara, CA)
Patent Number(s):
US 5631734
OSTI ID:
870962
Country of Publication:
United States
Language:
English

References (6)

Features and Applications of the Laser Scanning Microscope journal November 1990
Confocal compact scanning optical microscope based on compact disc technology journal January 1991
Development of microspot multi-analyte ratiometric immunoassay using dual fluorescent-labelled antibodies journal January 1989
An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy. journal July 1987
Multianalyte microspot immunoassay--microanalytical "compact disk" of the future journal November 1991
Design and use of a computer controlled confocal microscope for biological applications: Confocal microscope for biological applications journal January 1985

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Related Subjects

method
apparatus
detection
fluorescently
labeled
materials
marked
targets
bind
substrate
230
synthesized
polymer
sequences
locations
detected
exposing
selected
regions
light
source
100
detecting
photons
fluoresced
therefrom
repeating
steps
exposure
completely
examined
resulting
data
determine
binding
affinity
specific
light source
selected regions
resulting data
fluorescently labeled
selected region
specific polymer
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