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Title: Method for identifying biochemical and chemical reactions and micromechanical processes using nanomechanical and electronic signal identification

Abstract

A scanning probe microscope, such as an atomic force microscope (AFM) or a scanning tunneling microscope (STM), is operated in a stationary mode on a site where an activity of interest occurs to measure and identify characteristic time-varying micromotions caused by biological, chemical, mechanical, electrical, optical, or physical processes. The tip and cantilever assembly of an AFM is used as a micromechanical detector of characteristic micromotions transmitted either directly by a site of interest or indirectly through the surrounding medium. Alternatively, the exponential dependence of the tunneling current on the size of the gap in the STM is used to detect micromechanical movement. The stationary mode of operation can be used to observe dynamic biological processes in real time and in a natural environment, such as polymerase processing of DNA for determining the sequence of a DNA molecule.

Inventors:
 [1];  [1]
  1. (Berkeley, CA)
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
870903
Patent Number(s):
US 5620854
Assignee:
Regents of University Of California (Oakland, CA) LLNL
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
method; identifying; biochemical; chemical; reactions; micromechanical; processes; nanomechanical; electronic; signal; identification; scanning; probe; microscope; atomic; force; afm; tunneling; stm; operated; stationary; mode; site; activity; occurs; measure; identify; characteristic; time-varying; micromotions; caused; biological; mechanical; electrical; optical; physical; tip; cantilever; assembly; detector; transmitted; directly; indirectly; surrounding; medium; alternatively; exponential; dependence; current; size; gap; detect; movement; operation; observe; dynamic; time; natural; environment; polymerase; processing; dna; determining; sequence; molecule; scanning tunneling; tunneling microscope; chemical reaction; chemical reactions; scanning probe; dna molecule; electronic signal; tunneling current; atomic force; biological processes; force microscope; probe microscope; mechanical process; /435/250/436/

Citation Formats

Holzrichter, John F., and Siekhaus, Wigbert J. Method for identifying biochemical and chemical reactions and micromechanical processes using nanomechanical and electronic signal identification. United States: N. p., 1997. Web.
Holzrichter, John F., & Siekhaus, Wigbert J. Method for identifying biochemical and chemical reactions and micromechanical processes using nanomechanical and electronic signal identification. United States.
Holzrichter, John F., and Siekhaus, Wigbert J. Wed . "Method for identifying biochemical and chemical reactions and micromechanical processes using nanomechanical and electronic signal identification". United States. https://www.osti.gov/servlets/purl/870903.
@article{osti_870903,
title = {Method for identifying biochemical and chemical reactions and micromechanical processes using nanomechanical and electronic signal identification},
author = {Holzrichter, John F. and Siekhaus, Wigbert J.},
abstractNote = {A scanning probe microscope, such as an atomic force microscope (AFM) or a scanning tunneling microscope (STM), is operated in a stationary mode on a site where an activity of interest occurs to measure and identify characteristic time-varying micromotions caused by biological, chemical, mechanical, electrical, optical, or physical processes. The tip and cantilever assembly of an AFM is used as a micromechanical detector of characteristic micromotions transmitted either directly by a site of interest or indirectly through the surrounding medium. Alternatively, the exponential dependence of the tunneling current on the size of the gap in the STM is used to detect micromechanical movement. The stationary mode of operation can be used to observe dynamic biological processes in real time and in a natural environment, such as polymerase processing of DNA for determining the sequence of a DNA molecule.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1997},
month = {1}
}

Patent:

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