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Title: Ethanol production by recombinant hosts

Abstract

Novel plasmids comprising genes which code for the alcohol dehydrogenase and pyruvate decarboxylase are described. Also described are recombinant hosts which have been transformed with genes coding for alcohol dehydrogenase and pyruvate. By virtue of their transformation with these genes, the recombinant hosts are capable of producing significant amounts of ethanol as a fermentation product. Also disclosed are methods for increasing the growth of recombinant hosts and methods for reducing the accumulation of undesirable metabolic products in the growth medium of these hosts. Also disclosed are recombinant host capable of producing significant amounts of ethanol as a fermentation product of oligosaccharides and plasmids comprising genes encoding polysaccharases, in addition to the genes described above which code for the alcohol dehydrogenase and pyruvate decarboxylase. Further, methods are described for producing ethanol from oligomeric feedstock using the recombinant hosts described above. Also provided is a method for enhancing the production of functional proteins in a recombinant host comprising overexpressing an adhB gene in the host. Further provided are process designs for fermenting oligosaccharide-containing biomass to ethanol.

Inventors:
 [1];  [1];  [1];  [2];  [3];  [1];  [1]
  1. (Gainesville, FL)
  2. (Vicosa, BR)
  3. (Miyazaki, JP)
Publication Date:
Research Org.:
University of Florida (Gainesville, FL)
OSTI Identifier:
869925
Patent Number(s):
US 5424202
Assignee:
University of Florida (Gainesville, FL) ORO
DOE Contract Number:
FG05-86ER13574
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
ethanol; production; recombinant; hosts; novel; plasmids; comprising; genes; code; alcohol; dehydrogenase; pyruvate; decarboxylase; described; transformed; coding; virtue; transformation; capable; producing; significant; amounts; fermentation; product; disclosed; methods; increasing; growth; reducing; accumulation; undesirable; metabolic; products; medium; host; oligosaccharides; encoding; polysaccharases; addition; oligomeric; feedstock; provided; method; enhancing; functional; proteins; overexpressing; adhb; process; designs; fermenting; oligosaccharide-containing; biomass; fermentation product; comprising genes; plasmids comprising; ethanol production; producing ethanol; significant amounts; alcohol dehydrogenase; recombinant host; recombinant hosts; pyruvate decarboxylase; undesirable metabolic; metabolic products; significant amount; growth medium; novel plasmids; functional proteins; genes coding; genes encoding; process design; /435/

Citation Formats

Ingram, Lonnie O., Beall, David S., Burchhardt, Gerhard F. H., Guimaraes, Walter V., Ohta, Kazuyoshi, Wood, Brent E., and Shanmugam, Keelnatham T. Ethanol production by recombinant hosts. United States: N. p., 1995. Web.
Ingram, Lonnie O., Beall, David S., Burchhardt, Gerhard F. H., Guimaraes, Walter V., Ohta, Kazuyoshi, Wood, Brent E., & Shanmugam, Keelnatham T. Ethanol production by recombinant hosts. United States.
Ingram, Lonnie O., Beall, David S., Burchhardt, Gerhard F. H., Guimaraes, Walter V., Ohta, Kazuyoshi, Wood, Brent E., and Shanmugam, Keelnatham T. 1995. "Ethanol production by recombinant hosts". United States. doi:. https://www.osti.gov/servlets/purl/869925.
@article{osti_869925,
title = {Ethanol production by recombinant hosts},
author = {Ingram, Lonnie O. and Beall, David S. and Burchhardt, Gerhard F. H. and Guimaraes, Walter V. and Ohta, Kazuyoshi and Wood, Brent E. and Shanmugam, Keelnatham T.},
abstractNote = {Novel plasmids comprising genes which code for the alcohol dehydrogenase and pyruvate decarboxylase are described. Also described are recombinant hosts which have been transformed with genes coding for alcohol dehydrogenase and pyruvate. By virtue of their transformation with these genes, the recombinant hosts are capable of producing significant amounts of ethanol as a fermentation product. Also disclosed are methods for increasing the growth of recombinant hosts and methods for reducing the accumulation of undesirable metabolic products in the growth medium of these hosts. Also disclosed are recombinant host capable of producing significant amounts of ethanol as a fermentation product of oligosaccharides and plasmids comprising genes encoding polysaccharases, in addition to the genes described above which code for the alcohol dehydrogenase and pyruvate decarboxylase. Further, methods are described for producing ethanol from oligomeric feedstock using the recombinant hosts described above. Also provided is a method for enhancing the production of functional proteins in a recombinant host comprising overexpressing an adhB gene in the host. Further provided are process designs for fermenting oligosaccharide-containing biomass to ethanol.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = 1995,
month = 1
}

Patent:

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  • Novel plasmids comprising genes which code for the alcohol dehydrogenase and pyruvate decarboxylase are described. Also described are recombinant hosts which have been transformed with genes coding for alcohol dehydrogenase and pyruvate. By virtue of their transformation with these genes, the recombinant hosts are capable of producing significant amounts of ethanol as a fermentation product. Also disclosed are methods for increasing the growth of recombinant hosts and methods for reducing the accumulation of undesirable metabolic products in the growth medium of these hosts. Also disclosed are recombinant host capable of producing significant amounts of ethanol as a fermentation product ofmore » oligosaccharides and plasmids comprising genes encoding polysaccharases, in addition to the genes described above which code for the alcohol dehydrogenase and pyruvate decarboxylase. Further, methods are described for producing ethanol from oligomeric feedstock using the recombinant hosts described above. Also provided is a method for enhancing the production of functional proteins in a recombinant host comprising overexpressing an adhB gene in the host. Further provided are process designs for fermenting oligosaccharide-containing biomass to ethanol.« less
  • Non-recombinant bacteria that produce ethanol as the primary fermentation product, associated nucleic acids and polypeptides, methods for producing ethanol using the bacteria, and kits are disclosed.
  • The subject invention concerns the transformation of Gram-positive bacteria with heterologous genes which confer upon these microbes the ability to produce ethanol as a fermentation product. Specifically exemplified is the transformation of bacteria with genes, obtainable from Zymomonas mobilis, which encode pyruvate decarboxylase and alcohol dehydrogenase.
  • Disclosed are recombinant host cells suitable for degrading an oligosaccharide that have been optimized for growth and production of high yields of ethanol, and methods of making and using these cells. The invention further provides minimal media comprising urea-like compounds for economical production of ethanol by recombinant microorganisms. Recombinant host cells in accordance with the invention are modified by gene mutation to eliminate genes responsible for the production of unwanted products other than ethanol, thereby increasing the yield of ethanol produced from the oligosaccharides, relative to unmutated parent strains. The new and improved strains of recombinant bacteria are capable ofmore » superior ethanol productivity and yield when grown under conditions suitable for fermentation in minimal growth media containing inexpensive reagents. Systems optimized for ethanol production combine a selected optimized minimal medium with a recombinant host cell optimized for use in the selected medium. Preferred systems are suitable for efficient ethanol production by simultaneous saccharification and fermentation (SSF) using lignocellulose as an oligosaccharide source. The invention also provides novel isolated polynucleotide sequences, polypeptide sequences, vectors and antibodies.« less
  • The invention provides recombinant host cells containing at least one heterologous polynucleotide encoding a polysaccharase under the transcriptional control of a surrogate promoter capable of increasing the expression of the polysaccharase. In addition, the invention further provides such hosts with genes encoding secretory protein/s to facilitate the secretion of the expressed polysaccharase. Preferred hosts of the invention are ethanologenic and capable of carrying out simultaneous saccharification fermentation resulting in the production of ethanol from complex cellulose substrates.