skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Cloning vector

Abstract

A vector comprising a filamentous phage sequence containing a first copy of filamentous phage gene X and other sequences necessary for the phage to propagate is disclosed. The vector also contains a second copy of filamentous phage gene X downstream from a promoter capable of promoting transcription in a bacterial host. In a preferred form of the present invention, the filamentous phage is M13 and the vector additionally includes a restriction endonuclease site located in such a manner as to substantially inactivate the second gene X when a DNA sequence is inserted into the restriction site.

Inventors:
 [1];  [1]
  1. (Madison, WI)
Publication Date:
Research Org.:
University of Wisconsin
OSTI Identifier:
869677
Patent Number(s):
US 5376549
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI) CHO
DOE Contract Number:  
FG02-90ER61026
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
cloning; vector; comprising; filamentous; phage; sequence; containing; copy; sequences; propagate; disclosed; contains; downstream; promoter; capable; promoting; transcription; bacterial; host; preferred; form; m13; additionally; restriction; endonuclease; site; located; manner; substantially; inactivate; dna; inserted; bacterial host; dna sequence; preferred form; cloning vector; restriction site; filamentous phage; restriction endonuclease; vector comprising; /435/

Citation Formats

Guilfoyle, Richard A., and Smith, Lloyd M. Cloning vector. United States: N. p., 1994. Web.
Guilfoyle, Richard A., & Smith, Lloyd M. Cloning vector. United States.
Guilfoyle, Richard A., and Smith, Lloyd M. Sat . "Cloning vector". United States. https://www.osti.gov/servlets/purl/869677.
@article{osti_869677,
title = {Cloning vector},
author = {Guilfoyle, Richard A. and Smith, Lloyd M.},
abstractNote = {A vector comprising a filamentous phage sequence containing a first copy of filamentous phage gene X and other sequences necessary for the phage to propagate is disclosed. The vector also contains a second copy of filamentous phage gene X downstream from a promoter capable of promoting transcription in a bacterial host. In a preferred form of the present invention, the filamentous phage is M13 and the vector additionally includes a restriction endonuclease site located in such a manner as to substantially inactivate the second gene X when a DNA sequence is inserted into the restriction site.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1994},
month = {1}
}

Patent:

Save / Share: