Method to directly radiolabel antibodies for diagnostic imaging and therapy

Thakur, Mathew L

Method to directly radiolabel antibodies for diagnostic imaging and therapy

Patent · 31 December 1993

OSTI ID:869275

Thakur, Mathew L ^[1]

Cherry Hill, NJ

The invention is a novel method and kit for directly radiolabeling proteins such as antibodies or antibody fragments for diagnostic and therapeutic purposes. The method comprises incubating a protein-containing solution with a solution of sodium ascorbate; adding a required quantity of reduced radionuclide to the incubated protein. A kit is also provided wherein the protein and/or reducing agents may be in lyophilized form.

Research Organization:: Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA

Assignee:: Thomas Jefferson University (Philadelphia, PA)

Patent Number(s):: US 5308603

OSTI ID:: 869275

Country of Publication:: United States

Language:: English

References (7)

Serum stability and non-specific binding of technetium-99m labeled diaminodithiol for protein labeling Liang, F. H.; Virzi, F.; Hnatowich, D. J. International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology, Vol. 14, Issue 6 https://doi.org/10.1016/0883-2897(87)90025-0	journal	January 1987
The production of 99mTc-labeled conjugated antibodies using a cyclam-based bifunctional chelating agent Franz, J.; Volkert, W. A.; Barefield, E. K. International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology, Vol. 14, Issue 6 https://doi.org/10.1016/0883-2897(87)90027-4	journal	January 1987
The labeling of high affinity sites of antibodies with 99mTc Paik, Chang H.; Phan, Lan N. B.; Hong, Jack J. International Journal of Nuclear Medicine and Biology, Vol. 12, Issue 1 https://doi.org/10.1016/0047-0740(85)90004-X	journal	January 1985
Formation of99mTc-immunoglobulin G complexes free from radiocolloids, quality controlled by radioimmunoelectrophoresis Sundrehagen, Erling European Journal of Nuclear Medicine, Vol. 7, Issue 12 https://doi.org/10.1007/BF00571648	journal	January 1982
Site-specific radiolabeling of monoclonal antibodies with biotin/streptavidin Del Rosario, Renato B.; Wahl, Richard L. International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology, Vol. 16, Issue 5 https://doi.org/10.1016/0883-2897(89)90067-6	journal	January 1989
Dithiothreitol, a New Protective Reagent for SH Groups ^* Cleland, W. W. Biochemistry, Vol. 3, Issue 4 https://doi.org/10.1021/bi00892a002	journal	April 1964
A new method for protein labeling with 99mTc Blok, D.; Feitsma, R. I. J.; Wasser, M. N. J. M. International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology, Vol. 16, Issue 1 https://doi.org/10.1016/0883-2897(89)90209-2	journal	January 1989

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Related Subjects

/424/530/
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ascorbate
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diagnostic
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lyophilized
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quantity
radiolabel
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reduced
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reducing agent
reducing agents
required
sodium
solution
therapeutic
therapeutic purposes
therapy

Method to directly radiolabel antibodies for diagnostic imaging and therapy

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References (7)

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