Gene encoding plant asparagine synthetase

Coruzzi, Gloria M; Tsai, Fong-Ying

Gene encoding plant asparagine synthetase

Patent · 26 October 1993

OSTI ID:868982

Coruzzi, Gloria M ^[1]; Tsai, Fong-Ying ^[1]

New York, NY

The identification and cloning of the gene(s) for plant asparagine synthetase (AS), an important enzyme involved in the formation of asparagine, a major nitrogen transport compound of higher plants is described. Expression vectors constructed with the AS coding sequence may be utilized to produce plant AS; to engineer herbicide resistant plants, salt/drought tolerant plants or pathogen resistant plants; as a dominant selectable marker; or to select for novel herbicides or compounds useful as agents that synchronize plant cells in culture. The promoter for plant AS, which directs high levels of gene expression and is induced in an organ specific manner and by darkness, is also described. The AS promoter may be used to direct the expression of heterologous coding sequences in appropriate hosts.

View Patent

Assignee:: Trustees of Rockefeller University (New York, NY)

Patent Number(s):: US 5256558

Application Number:: 07/514,816

OSTI ID:: 868982

Country of Publication:: United States

Language:: English

References (30)

The purification and properties of glutamine-dependent asparagine synthetase isolated from Lupinus albus Lea, Peter John; Fowden, Leslie Proceedings of the Royal Society of London. Series B. Biological Sciences, Vol. 192, Issue 1106, p. 13-26 https://doi.org/10.1098/rspb.1975.0148	journal	December 1975
Nucleotide sequence of the asnA gene coding for asparagine synthetase of E.coli K-12 Nakamura, Masataka; Yamada, Masao; Hirota, Yukinori Nucleic Acids Research, Vol. 9, Issue 18 https://doi.org/10.1093/nar/9.18.4669	journal	January 1981
Expression of Human Asparagine Synthetase in Escherichia Coli Van Heeke, G.; Schuster, S. M. Journal of Biological Chemistry, Vol. 264, Issue 10 https://doi.org/10.1016/S0021-9258(18)83573-6	journal	April 1989
Glutamine-dependent asparagine synthetase from Lupinus luteus Rognes, Sven Erik Phytochemistry, Vol. 14, Issue 9 https://doi.org/10.1016/0031-9422(75)83108-6	journal	September 1975
Nitrogen Metabolism in Canavalia ensiformis (L.) DC. Loyola-Vargas, Víctor M.; Yáñez, Alicia; Caldera, Juan Journal of Plant Physiology, Vol. 132, Issue 3 https://doi.org/10.1016/S0176-1617(88)80107-X	journal	April 1988
Use of the Escherichia coli gene for asparagine synthetase as a selective marker in a shuttle vector capable of dominant transfection and amplification in animal cells. Cartier, M.; Chang, M. W.; Stanners, C. P. Molecular and Cellular Biology, Vol. 7, Issue 5 https://doi.org/10.1128/MCB.7.5.1623	journal	May 1987
Asparagine Synthesis in Pea Leaves, and the Occurrence of an Asparagine Synthetase Inhibitor Joy, Kenneth W.; Ireland, Robert J.; Lea, Peter J. Plant Physiology, Vol. 73, Issue 1 https://doi.org/10.1104/pp.73.1.165	journal	September 1983
Genetic and biomedical studies demonstrating a second gene coding for asparagine synthetase in Escherichia coli Humbert, R.; Simoni, R. D. Journal of Bacteriology, Vol. 142, Issue 1 https://doi.org/10.1128/jb.142.1.212-220.1980	journal	April 1980
Asparagine Biosynthesis in Soybean Nodules Huber, Thomas A.; Streeter, John G. Plant Physiology, Vol. 74, Issue 3 https://doi.org/10.1104/pp.74.3.605	journal	March 1984
Molecular cloning of a cDNA for Chinese hamster ovary asparagine synthetase Ray, Peter N.; Siminovitch, Louis; Andrulis, Irene L. Gene, Vol. 30, Issue 1-3 https://doi.org/10.1016/0378-1119(84)90098-2	journal	October 1984
Multiple forms of rat liver L-asparagine synthetase Markin, Rodney S.; Schuster, Sheldon M. Biochemical and Biophysical Research Communications, Vol. 88, Issue 2 https://doi.org/10.1016/0006-291X(79)92088-6	journal	May 1979
Sulphamethoxazole Prophylaxis in the Otitis-Prone Child No authors listed The Pediatric Infectious Disease Journal, Vol. 2, Issue 1 https://doi.org/10.1097/00006454-198301000-00020	journal	January 1983
Monoclonal antibodies specific for bovine pancreatic asparagine synthetase. Production and use in structural studies. Pfeiffer, N. E.; Mehlhaff, P. M.; Wylie, D. E. Journal of Biological Chemistry, Vol. 261, Issue 4 https://doi.org/10.1016/S0021-9258(17)36029-5	journal	February 1986
Partial purification and properties of l-asparagine synthetase from mouse pancreas Milman, Harry A.; Cooney, David A. Biochemical Journal, Vol. 181, Issue 1 https://doi.org/10.1042/bj1810051	journal	July 1979
Some molecular properties of asparagine synthetase from rat liver Hongo, Shigeki; Sato, Tsuneo Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 742, Issue 3 https://doi.org/10.1016/0167-4838(83)90265-0	journal	February 1983
Two Asparagine Synthetases in Saccharomyces cerevisiae Ramos, Fernando; Wiame, Jean-Marie European Journal of Biochemistry, Vol. 108, Issue 2 https://doi.org/10.1111/j.1432-1033.1980.tb04732.x	journal	July 1980
Purification and properties of asparagine synthetase from soybean root nodules Huber, Thomas A.; Streeter, John G. Plant Science, Vol. 42, Issue 1 https://doi.org/10.1016/0168-9452(85)90022-6	journal	December 1985
Topographical separation of the catalytic sites of asparagine synthetase explored with monoclonal antibodies. Pfeiffer, N. E.; Mehlhaff, P. M.; Wylie, D. E. Journal of Biological Chemistry, Vol. 262, Issue 24 https://doi.org/10.1016/S0021-9258(18)60845-2	journal	August 1987
Purification and properties of asparagine synthetase from rat liver Hongo, Shigeki; Matsumoto, Takako; Sato, Tsuneo Biochimica et Biophysica Acta (BBA) - Enzymology, Vol. 522, Issue 1 https://doi.org/10.1016/0005-2744(78)90342-X	journal	January 1978
L-Asparagine auxotrophs of Saccharomyces cerevisiae: genetic and phenotypic characterization Jones, G. E. Journal of Bacteriology, Vol. 134, Issue 1 https://doi.org/10.1128/jb.134.1.200-207.1978	journal	April 1978
Glutamine synthetase genes of pea encode distinct polypeptides which are differentially expressed in leaves, roots and nodules. Tingey, S. V.; Walker, E. L.; Coruzzi, G. M. The EMBO Journal, Vol. 6, Issue 1, p. 1-9 https://doi.org/10.1002/j.1460-2075.1987.tb04710.x	journal	January 1987
Subcellular location of enzymes of ammonia assimilation and asparagine synthesis in root nodules of Lupinus albus L. Shelp, B. J.; Atkins, C. A. Plant Science Letters, Vol. 36, Issue 3 https://doi.org/10.1016/0304-4211(84)90173-1	journal	October 1984
Dark-induced and organ-specific expression of two asparagine synthetase genes in Pisum sativum. Tsai, F. Y.; Coruzzi, G. M. The EMBO Journal, Vol. 9, Issue 2 https://doi.org/10.1002/j.1460-2075.1990.tb08114.x	journal	February 1990
Isolation of human cDNAs for asparagine synthetase and expression in Jensen rat sarcoma cells. Andrulis, I. L.; Chen, J.; Ray, P. N. Molecular and Cellular Biology, Vol. 7, Issue 7 https://doi.org/10.1128/MCB.7.7.2435	journal	July 1987
Eigenkompensation oder Fremdkompensation Schuster, Falko Countertrade professionell https://doi.org/10.1007/978-3-322-93015-6_13	book	January 1988
Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector Herrera-Estrella, L.; Van den Broeck, G.; Maenhaut, R. Nature, Vol. 310, Issue 5973 https://doi.org/10.1038/310115a0	journal	July 1984
Isopropyl-N(3-chlorophenyl) carbamate (CIPC) induced chromosomal loss in soybean: a new tool for plant somatic cell genetics Roth, E. J.; Lark, K. G. Theoretical and Applied Genetics, Vol. 68, Issue 5 https://doi.org/10.1007/BF00254811	journal	August 1984
Purification of rat liver asparagine synthetase by affinity chromatography on reactive blue 2-agarose Hongo, Shigeki; Sato, Tsuneo Analytical Biochemistry, Vol. 114, Issue 1 https://doi.org/10.1016/0003-2697(81)90468-1	journal	June 1981
Assignment of structural gene for asparagine synthetase to human chromosome 7 Arfin, Stuart M.; Cirullo, Ronald E.; Arredondo-Vega, F. X. Somatic Cell Genetics, Vol. 9, Issue 5 https://doi.org/10.1007/BF01574256	journal	September 1983
Characterization of single step albizziin-resistant Chinese hamster ovary cell lines with elevated levels of asparagine synthetase activity. Andrulis, I. L.; Evans-Blackler, S.; Siminovitch, L. Journal of Biological Chemistry, Vol. 260, Issue 12 https://doi.org/10.1016/S0021-9258(17)39638-2	journal	June 1985

Similar Records

Gene encoding plant asparagine synthetase

Patent · 1993 · OSTI ID:5349247

Organization and expression of the cell cycle gene, ts11, that encodes asparagine synthetase

Journal Article · 1989 · Molecular and Cellular Biology; (USA) · OSTI ID:5601518

Altered expression of asparagine synthetase mRNA in human leukemic and carcinoma cell lines

Journal Article · 1994 · American Journal of Human Genetics · OSTI ID:134631

Related Subjects

/435/999/
agents
appropriate
asparagine
asparagine synthetase
cells
cloning
coding
coding sequence
coding sequences
compound
compounds
compounds useful
constructed
culture
darkness
described
direct
directs
dominant
drought
encoding
engineer
enzyme
expression
expression vector
expression vectors
formation
herbicide
herbicides
heterologous
hosts
identification
induced
involved
levels
major
manner
marker
nitrogen
novel
organ
pathogen
plant
plant asparagine
plant cell
plant cells
plants
produce
promoter
resistant
resistant plants
salt
select
selectable
sequence
sequences
specific
specific manner
synchronize
synthetase
tolerant
transport
useful
utilized
vectors

Gene encoding plant asparagine synthetase

Citation Formats

References (30)

Similar Records

Related Subjects