Fiber optic-based regenerable biosensor

Patent · Fri Jan 01 00:00:00 EST 1993

OSTI ID:868619

Sepaniak, Michael J ^[1]; Vo-Dinh, Tuan ^[1]

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1. Knoxville, TN

A fiber optic-based regenerable biosensor. The biosensor is particularly suitable for use in microscale work in situ. In one embodiment, the biosensor comprises a reaction chamber disposed adjacent the distal end of a waveguide and adapted to receive therein a quantity of a sample containing an analyte. Leading into the chamber is a plurality of capillary conduits suitable for introducing into the chamber antibodies or other reagents suitable for selective interaction with a predetermined analyte. Following such interaction, the contents of the chamber may be subjected to an incident energy signal for developing fluorescence within the chamber that is detectable via the optical fiber and which is representative of the presence, i.e. concentration, of the selected analyte. Regeneration of the biosensor is accomplished by replacement of the reagents and/or the analyte, or a combination of these, at least in part via one or more of the capillary conduits. The capillary conduits extend from their respective terminal ends that are in fluid communication with the chamber, away from the chamber to respective location(s) remote from the chamber thereby permitting in situ location of the chamber and remote manipulation and/or analysis of the activity with the chamber.

• Research Organization: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
• Assignee: University of Tennessee Research Corporation (Knoxville, TN)
• Patent Number(s): US 5176881
• OSTI ID: 868619
• Country of Publication: United States
• Language: English

Related Subjects

fiber
optic-based
regenerable
biosensor
particularly
suitable
microscale
situ
embodiment
comprises
reaction
chamber
disposed
adjacent
distal
waveguide
adapted
receive
therein
quantity
sample
containing
analyte
leading
plurality
capillary
conduits
introducing
antibodies
reagents
selective
interaction
predetermined
following
contents
subjected
incident
energy
signal
developing
fluorescence
detectable
via
optical
representative
presence
concentration
selected
regeneration
accomplished
replacement
combination
extend
respective
terminal
fluid
communication
location
remote
permitting
manipulation
analysis
activity
chamber disposed
incident energy
sample containing
disposed adjacent
particularly suitable
reaction chamber
fiber optic
optical fiber
fluid communication
sensor comprises
remote manipulation
biosensor comprises
regenerable biosensor
reagents suitable
optic-based regenerable
fiber optic-based
/422/356/600/