Multiplex analysis of DNA

Patent · 31 December 1991

OSTI ID:868474

Church, George M ^[1]; Kieffer-Higgins, Stephen ^[2]

---

1. Boston, MA
2. Dorchester, MA

This invention features vectors and a method for sequencing DNA. The method includes the steps of: a) ligating the DNA into a vector comprising a tag sequence, the tag sequence includes at least 15 bases, wherein the tag sequence will not hybridize to the DNA under stringent hybridization conditions and is unique in the vector, to form a hybrid vector, b) treating the hybrid vector in a plurality of vessels to produce fragments comprising the tag sequence, wherein the fragments differ in length and terminate at a fixed known base or bases, wherein the fixed known base or bases differs in each vessel, c) separating the fragments from each vessel according to their size, d) hybridizing the fragments with an oligonucleotide able to hybridize specifically with the tag sequence, and e) detecting the pattern of hybridization of the tag sequence, wherein the pattern reflects the nucleotide sequence of the DNA.

• Research Organization: Harvard University
• DOE Contract Number: FG02-87ER60565
• Assignee: President and Fellows of Harvard College (Cambridge, MA)
• Patent Number(s): US 5149625
• OSTI ID: 868474
• Country of Publication: United States
• Language: English

Related Subjects

/435/436/
15
according
analysis
base
bases
comprising
conditions
detecting
differs
dna
features
fixed
form
fragments
hybrid
hybridization
hybridize
hybridizing
length
ligating
method
multiplex
nucleotide
nucleotide sequence
oligonucleotide
pattern
plurality
produce
reflects
separating
sequence
sequencing
sequencing dna
size
specifically
steps
stringent
tag
tag sequence
terminate
treating
unique
vector
vector comprising
vectors
vessel
vessels