DNA Sequencing apparatus

Tabor, Stanley; Richardson, Charles C

Title: DNA Sequencing apparatus

Patent · Wed Jan 01 00:00:00 EST 1992

OSTI ID:868349

Tabor, Stanley ^[1]; Richardson, Charles C ^[2]

Cambridge, MA
Chestnut Hill, MA

An automated DNA sequencing apparatus having a reactor for providing at least two series of DNA products formed from a single primer and a DNA strand, each DNA product of a series differing in molecular weight and having a chain terminating agent at one end; separating means for separating the DNA products to form a series bands, the intensity of substantially all nearby bands in a different series being different, band reading means for determining the position an This invention was made with government support including a grant from the U.S. Public Health Service, contract number AI-06045. The U.S. government has certain rights in the invention.

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Research Organization:: Harvard University

DOE Contract Number:: FG02-88ER60688

Assignee:: President and Fellows of Harvard College (Cambridge, MA)

Patent Number(s):: US 5122345

OSTI ID:: 868349

Country of Publication:: United States

Language:: English

References (60)

Structure-independent nucleotide sequence analysis. Mills, D. R.; Kramer, F. R. Proceedings of the National Academy of Sciences, Vol. 76, Issue 5 https://doi.org/10.1073/pnas.76.5.2232	journal	May 1979
Studies on Polynucleotides van de Sande, Johan H.; Loewen, Peter C.; Khorana, H. Gobind Journal of Biological Chemistry, Vol. 247, Issue 19 https://doi.org/10.1016/S0021-9258(19)44775-3	journal	October 1972
Metal activation of DNA synthesis Sirover, Michael A.; Loeb, Lawrence A. Biochemical and Biophysical Research Communications, Vol. 70, Issue 3 https://doi.org/10.1016/0006-291X(76)90664-1	journal	June 1976
On the fidelity of DNA replication. Effect of metal activators during synthesis with avian myeloblastosis virus DNA polymerase. Sirover, M. A.; Loeb, L. A. Journal of Biological Chemistry, Vol. 252, Issue 11 https://doi.org/10.1016/S0021-9258(17)40295-X	journal	June 1977
On the fidelity of DNA replication. Metal activation of Escherichia coli DNA polymerase I. Sirover, M. A.; Dube, D. K.; Loeb, L. A. Journal of Biological Chemistry, Vol. 254, Issue 1 https://doi.org/10.1016/S0021-9258(17)30278-8	journal	January 1979
[57] sequencing DNA with dideoxyribonucleotides as chain terminators: Hints and strategies for big projects Barnes, Wayne M. Methods in Enzymology https://doi.org/10.1016/0076-6879(87)52060-2	book	January 1987
[58] DNA sequence analysis by primed synthesis Smith, Andrew J. H. https://doi.org/10.1016/S0076-6879(80)65060-5	book	January 1980
The differential effect of magnesium and manganese ions on the synthesis of poly (dGd · C) and Micrococcus luteus DNA by Micrococcus luteus DNA polymerase Litman, Rose M. Journal of Molecular Biology, Vol. 61, Issue 1 https://doi.org/10.1016/0022-2836(71)90203-8	journal	October 1971
On the enzymatic basis for mutagenesis by manganese. Goodman, M. F.; Keener, S.; Guidotti, S. Journal of Biological Chemistry, Vol. 258, Issue 6 https://doi.org/10.1016/S0021-9258(18)32685-1	journal	March 1983
Bacteriophage T7 DNA replication. Synthesis of lagging strands in a reconstituted system using purified proteins. Engler, M. J.; Richardson, C. C. Journal of Biological Chemistry, Vol. 258, Issue 18 https://doi.org/10.1016/S0021-9258(17)44403-6	journal	September 1983
Use of DNA Polymerase I Primed by a Synthetic Oligonucleotide to Determine a Nucleotide Sequence in Phage f1 DNA Sanger, F.; Donelson, J. E.; Coulson, A. R. Proceedings of the National Academy of Sciences, Vol. 70, Issue 4 https://doi.org/10.1073/pnas.70.4.1209	journal	April 1973
Rapid sequence determination of late simian virus 40 16S mRNA leader by using inhibitors of reverse transcriptase. Bina-Stein, M.; Thoren, M.; Salzman, N. Proceedings of the National Academy of Sciences, Vol. 76, Issue 2 https://doi.org/10.1073/pnas.76.2.731	journal	February 1979
Protein structures: Two for the price of one Harrison, Stephen C. Nature, Vol. 313, Issue 6005 https://doi.org/10.1038/313736a0	journal	February 1985
An Improved Purification Method and a Physical Characterization of Phage T7 DNA Polymerase Randahl, Hakan; SlabÝ, Ivan; Holmgren, Arne European Journal of Biochemistry, Vol. 128, Issue 2-3 https://doi.org/10.1111/j.1432-1033.1982.tb06984.x	journal	November 1982
Replication of duplex DNA by bacteriophage T7 DNA polymerase and gene 4 protein is accompanied by hydrolysis of nucleoside 5'-triphosphates. Kolodner, R.; Richardson, C. C. Proceedings of the National Academy of Sciences, Vol. 74, Issue 4 https://doi.org/10.1073/pnas.74.4.1525	journal	April 1977
Effect of metal cations on misincorporation by E. coli DNA polymerases Miyaki, Michiko; Murata, Ikuko; Osabe, Michiko Biochemical and Biophysical Research Communications, Vol. 77, Issue 3 https://doi.org/10.1016/S0006-291X(77)80056-9	journal	August 1977
Effect of Variations in the Conditions of DNA Synthesis upon the Accuracy of DNA Replication Razzaki, Tashmeem; Sinha, Navin K. Genetic Consequences of Nucleotide Pool Imbalance https://doi.org/10.1007/978-1-4613-2449-2_11	book	January 1985
Supercoiled plasmid sequencing one buffer for template denaturation, primer annealing, and synthesis of labeled strands by reverse transcriptase Agellon, L. B.; Chen, T. T. Gene Analysis Techniques, Vol. 3, Issue 5 https://doi.org/10.1016/0735-0651(86)90008-7	journal	September 1986
New sequencers to take on the genome Roberts, L. Science, Vol. 238, Issue 4825 https://doi.org/10.1126/science.3659916	journal	October 1987
Initiation of DNA replication at the primary origin of bacteriophage T7 by purified proteins. Site and direction of initial DNA synthesis. Fuller, C. W.; Richardson, C. C. Journal of Biological Chemistry, Vol. 260, Issue 5 https://doi.org/10.1016/S0021-9258(18)89490-X	journal	March 1985
[56] DNA sequencing: Chemical methods Ambrose, Barbara J. B.; Pless, Reynaldo C. Methods in Enzymology https://doi.org/10.1016/0076-6879(87)52059-6	book	January 1987
Sequence analysis of end-labeled DNA fragments by solvolysis in hot aqueous piperidine solutions Ambrose, Barbara J. B.; Pless, Reynaldo C.; Ayers, Mary E. Analytical Biochemistry, Vol. 169, Issue 1 https://doi.org/10.1016/0003-2697(88)90266-7	journal	February 1988
Analysis of DNA sequences using a single chemical cleavage procedure Ambrose, Barbara J. B.; Pless, Reynaldo C. Biochemistry, Vol. 24, Issue 22 https://doi.org/10.1021/bi00343a025	journal	October 1985
A new method for sequencing DNA. Maxam, A. M.; Gilbert, W. Proceedings of the National Academy of Sciences, Vol. 74, Issue 2 https://doi.org/10.1073/pnas.74.2.560	journal	February 1977
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase Saiki, R. K.; Gelfand, D. H; Stoffel, S. Science, Vol. 239, Issue 4839, p. 487-491 https://doi.org/10.1126/science.239.4839.487	journal	January 1988
Escherichia coli DNA polymerases II and III: Activation by magnesium or by manganous ions Helfman, William B.; Hendler, Sheldon S.; Smith, Douglas W. Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, Vol. 447, Issue 2 https://doi.org/10.1016/0005-2787(76)90341-5	journal	October 1976
Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP Ollis, D. L.; Brick, P.; Hamlin, R. Nature, Vol. 313, Issue 6005 https://doi.org/10.1038/313762a0	journal	February 1985
Template–dependent variation in the relative fidelity of DNA polymerase I of Escherichia coli in the presence of Mg ²⁺ versus Mn ²⁺ Hillebrand, Greg G.; Beattie, Kenneth Nucleic Acids Research, Vol. 12, Issue 7 https://doi.org/10.1093/nar/12.7.3173	journal	January 1984
Processive nature of reverse transcription by avian myeloblastosis virus deoxyribonucleic acid polymerase Gregerson, Dale S.; Albert, Joseph; Reid, Ted W. Biochemistry, Vol. 19, Issue 2 https://doi.org/10.1021/bi00543a008	journal	January 1980
On the fidelity of DNA polymerase α: the influence of α-thio dNTPs, Mn ²⁺ and mismatch repair Rienitz, Axel; Grosse, Frank; Blöcker, Helmut Nucleic Acids Research, Vol. 13, Issue 15 https://doi.org/10.1093/nar/13.15.5685	journal	January 1985
[11] The kinetics and processivity of nucleic acid polymerases McClure, William R.; Chow, Yvonne Methods in Enzymology https://doi.org/10.1016/S0076-6879(80)64013-0	book	January 1980
Sequencing of large double-stranded DNA using the dideoxy sequencing technique Hong, G. F. Bioscience Reports, Vol. 2, Issue 11 https://doi.org/10.1007/BF01114897	journal	November 1982
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing Sanger, F.; Coulson, A. R.; Barrell, B. G. Journal of Molecular Biology, Vol. 143, Issue 2 https://doi.org/10.1016/0022-2836(80)90196-5	journal	October 1980
Colorimetric-detected DNA sequencing Beck, Stephan Analytical Biochemistry, Vol. 164, Issue 2 https://doi.org/10.1016/0003-2697(87)90526-4	journal	August 1987
Site-specific analysis of drug interactions and damage in DNA using sequencing techniques Wilkins, R. J. Analytical Biochemistry, Vol. 147, Issue 2 https://doi.org/10.1016/0003-2697(85)90271-4	journal	June 1985
Effect of manganese(2+) ions on the in vitro activity of human deoxyribonucleic acid polymerase .beta. Wang, Teresa Shu-Fong; Eichler, Duane C.; Korn, David Biochemistry, Vol. 16, Issue 22 https://doi.org/10.1021/bi00641a029	journal	November 1977
Genomic sequencing. Church, G. M.; Gilbert, W. Proceedings of the National Academy of Sciences, Vol. 81, Issue 7 https://doi.org/10.1073/pnas.81.7.1991	journal	April 1984
DNA sequencing by partial ribosubstitution Barnes, Wayne M. Journal of Molecular Biology, Vol. 119, Issue 1 https://doi.org/10.1016/0022-2836(78)90271-1	journal	February 1978
Characterization of bacteriophage T7 DNA polymerase purified to homogeneity by antithioredoxin immunoadsorbent chromatography. Nordström, B.; Randahl, H.; Slaby, I. Journal of Biological Chemistry, Vol. 256, Issue 6 https://doi.org/10.1016/S0021-9258(19)69731-0	journal	March 1981
Rapid and easy sequencing of large linear double-stranded DNA and supercoiled plasmid DNA Zagursky, Robert J.; Baumeister, Kirk; Lomax, Nancy Gene Analysis Techniques, Vol. 2, Issue 5 https://doi.org/10.1016/0735-0651(85)90011-1	journal	September 1985
Studies on the mechanism of Escherichia coli DNA polymerase I large fragment. Chain termination and modulation by polynucleotides. Detera, S. D.; Wilson, S. H. Journal of Biological Chemistry, Vol. 257, Issue 16 https://doi.org/10.1016/S0021-9258(18)34139-5	journal	August 1982
Metal-induced infidelity of DNA synthesis Zakour, R. A.; Tkeshelashvili, L. K.; Shearman, C. W. Journal of Cancer Research and Clinical Oncology, Vol. 99, Issue 1-2 https://doi.org/10.1007/BF00412453	journal	February 1981
DNA and RNA sequence determination based on phosphorothioate chemistry Gish, G.; Eckstein, F. Science, Vol. 240, Issue 4858 https://doi.org/10.1126/science.2453926	journal	June 1988
Domain of E. coli DNA polymerase I showing sequence homology to T7 DNA polymerase Ollis, D. L.; Kline, C.; Steitz, T. A. Nature, Vol. 313, Issue 6005 https://doi.org/10.1038/313818a0	journal	February 1985
DNA sequencing with chain-terminating inhibitors Sanger, F.; Nicklen, S.; Coulson, A. R. Proceedings of the National Academy of Sciences, Vol. 74, Issue 12 https://doi.org/10.1073/pnas.74.12.5463	journal	December 1977
Enzymatic Synthesis of Deoxyribonucleic Acid Deutscher, M. P.; Kornberg, A. Journal of Biological Chemistry, Vol. 244, Issue 11 https://doi.org/10.1016/S0021-9258(18)91724-2	journal	June 1969
A method for sequencing restriction fragments with dideoxynucleoside triphosphates Maat, Jan; Smith, Andrew J. H. Nucleic Acids Research, Vol. 5, Issue 12 https://doi.org/10.1093/nar/5.12.4537	journal	January 1978
Nucleotide sequence of the thioredoxin gene from Escherichia coli Höög, Jan-Olov; von Bahr-Lindström, Hedvig; Josephson, Staffan Bioscience Reports, Vol. 4, Issue 11 https://doi.org/10.1007/BF01116889	journal	November 1984
On the fidelity of DNA replication. Effect of divalent metal ion activators and deoxyrionucleoside triphosphate pools on in vitro mutagenesis. Kunkel, T. A.; Loeb, L. A. Journal of Biological Chemistry, Vol. 254, Issue 13 https://doi.org/10.1016/S0021-9258(18)50474-9	journal	August 1979
One-lane sequence analysis of oligodeoxyribonucleotides Ambrose, Barbara J. B.; Castro, Miguel M.; Pless, Reynaldo C. Analytical Biochemistry, Vol. 159, Issue 1 https://doi.org/10.1016/0003-2697(86)90302-7	journal	November 1986
Transcription from bacteriophage T7 and SP6 RNA polymerase promoters in the presence of 3'-deoxyribonucleoside 5'-triphosphate chain terminators Axelrod, Vladimir D.; Kramer, Fred Russell Biochemistry, Vol. 24, Issue 21 https://doi.org/10.1021/bi00342a005	journal	October 1985
Characterization of β-thalassaemia mutations using direct genomic sequencing of amplified single copy DNA Wong, Corinne; Dowling, Carol E.; Saiki, Randall K. Nature, Vol. 330, Issue 6146 https://doi.org/10.1038/330384a0	journal	November 1987
The effect of magnesium and manganese ions on the structure and template activity for reverse transcriptase of polyribocytidylate and its 2'-o-methyl derivative Vamvakopoulos, Nikos C.; Vournakis, John N.; Marcus, Stuart L. Nucleic Acids Research, Vol. 4, Issue 10 https://doi.org/10.1093/nar/4.10.3589	journal	January 1977
DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Tabor, S.; Richardson, C. C. Proceedings of the National Academy of Sciences, Vol. 84, Issue 14 https://doi.org/10.1073/pnas.84.14.4767	journal	July 1987
Nucleotide Sequencing of DNA: Preliminary Characterization of the Products of Specific Cleavages at Guanine, Cytosine, or Adenine Residues Salser, W.; Fry, K.; Brunk, C. Proceedings of the National Academy of Sciences, Vol. 69, Issue 1 https://doi.org/10.1073/pnas.69.1.238	journal	January 1972
Dideoxy DNA sequencing with end-labeled oligonucleotide primers McGraw, Royal A. Analytical Biochemistry, Vol. 143, Issue 2 https://doi.org/10.1016/0003-2697(84)90666-3	journal	December 1984
Inhibition of deoxyribonucleic acid polymerases of human leukemic leukocytes by 2',3'-dideoxythymidine triphosphate Allaudeen, H. S. Biochemical Pharmacology, Vol. 29, Issue 8 https://doi.org/10.1016/0006-2952(80)90410-4	journal	April 1980
A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase Sanger, F.; Coulson, A. R. Journal of Molecular Biology, Vol. 94, Issue 3 https://doi.org/10.1016/0022-2836(75)90213-2	journal	May 1975
T7 DNA polymerase in automated dideoxy sequencing Kristensen, Tom; Voss, Hartmut; Schwager, Christian Nucleic Acids Research, Vol. 16, Issue 8 https://doi.org/10.1093/nar/16.8.3487	journal	January 1988
Differences in the effects of manganese and magnesium on initiation and elongation in the RNA polymerase I reaction Nagamine, Yoshikuni; Mizuno, Den'ichi; Natori, Sunji Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, Vol. 519, Issue 2 https://doi.org/10.1016/0005-2787(78)90097-7	journal	July 1978

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dna
sequencing
apparatus
automated
reactor
providing
series
products
formed
single
primer
strand
product
differing
molecular
weight
chain
terminating
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separating
means
form
bands
intensity
substantially
nearby
band
reading
determining
position
government
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public
health
service
contract
ai-06045
rights
dna strand
molecular weight
dna sequencing
government support
support including
sequencing apparatus
products formed
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Title: DNA Sequencing apparatus

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References (60)

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