Quantitative luminescence imaging system

Patent · Mon Jan 01 00:00:00 EST 1990

OSTI ID:867493

Erwin, David N ^[1]; Kiel, Johnathan L ^[1]; Batishko, Charles R ^[2]; Stahl, Kurt A ^[3]

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1. San Antonio, TX
2. West Richland, WA
3. Richland, WA

The QLIS images and quantifies low-level chemiluminescent reactions in an electromagnetic field. It is capable of real time nonperturbing measurement and simultaneous recording of many biochemical and chemical reactions such as luminescent immunoassays or enzyme assays. The system comprises image transfer optics, a low-light level digitizing camera with image intensifying microchannel plates, an image process or, and a control computer. The image transfer optics may be a fiber image guide with a bend, or a microscope, to take the light outside of the RF field. Output of the camera is transformed into a localized rate of cumulative digitalized data or enhanced video display or hard-copy images. The system may be used as a luminescent microdosimetry device for radiofrequency or microwave radiation, as a thermal dosimeter, or in the dosimetry of ultra-sound (sonoluminescence) or ionizing radiation. It provides a near-real-time system capable of measuring the extremely low light levels from luminescent reactions in electromagnetic fields in the areas of chemiluminescence assays and thermal microdosimetry, and is capable of near-real-time imaging of the sample to allow spatial distribution analysis of the reaction. It can be used to instrument three distinctly different irradiation configurations, comprising (1) RF waveguide irradiation of a small Petri-dish-shaped sample cell, (2) RF irradiation of samples in a microscope for the microscopie imaging and measurement, and (3) RF irradiation of small to human body-sized samples in an anechoic chamber.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
• DOE Contract Number: AC06-76RL01830
• Assignee: United States of America as represented by Secretary of Air (Washington, DC)
• Patent Number(s): US 4948975
• OSTI ID: 867493
• Country of Publication: United States
• Language: English

Related Subjects

quantitative
luminescence
imaging
qlis
images
quantifies
low-level
chemiluminescent
reactions
electromagnetic
field
capable
time
nonperturbing
measurement
simultaneous
recording
biochemical
chemical
luminescent
immunoassays
enzyme
assays
comprises
image
transfer
optics
low-light
level
digitizing
camera
intensifying
microchannel
plates
process
control
computer
fiber
guide
bend
microscope
light
outside
rf
output
transformed
localized
rate
cumulative
digitalized
data
enhanced
video
display
hard-copy
microdosimetry
device
radiofrequency
microwave
radiation
thermal
dosimeter
dosimetry
ultra-sound
sonoluminescence
ionizing
provides
near-real-time
measuring
extremely
levels
fields
chemiluminescence
sample
allow
spatial
distribution
analysis
reaction
instrument
distinctly
irradiation
configurations
comprising
waveguide
petri-dish-shaped
cell
samples
microscopie
human
body-sized
anechoic
chamber
channel plate
control computer
electromagnetic fields
electromagnetic field
microwave radiation
magnetic field
magnetic fields
chemical reaction
ionizing radiation
microchannel plate
chemical reactions
video display
microchannel plates
rf field
light level
anechoic chamber
/250/422/