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Title: Computer controlled fluorometer device and method of operating same

Abstract

A computer controlled fluorometer device and method of operating same, said device being made to include a pump flash source and a probe flash source and one or more sample chambers in combination with a light condenser lens system and associated filters and reflectors and collimators, as well as signal conditioning and monitoring means and a programmable computer means and a software programmable source of background irradiance that is operable according to the method of the invention to rapidly, efficiently and accurately measure photosynthetic activity by precisely monitoring and recording changes in fluorescence yield produced by a controlled series of predetermined cycles of probe and pump flashes from the respective probe and pump sources that are controlled by the computer means.

Inventors:
 [1];  [2]
  1. (Shoreham, NY)
  2. (Stony Brook, NY)
Publication Date:
Research Org.:
ASSOC UNIVERSITIES INC
OSTI Identifier:
867463
Patent Number(s):
US 4942303
Assignee:
Associated Universities, Inc. (Washington, DC) BNL
DOE Contract Number:
AC02-76CH00016
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
computer; controlled; fluorometer; device; method; operating; pump; flash; source; probe; sample; chambers; combination; light; condenser; lens; associated; filters; reflectors; collimators; signal; conditioning; monitoring; means; programmable; software; background; irradiance; operable; according; rapidly; efficiently; accurately; measure; photosynthetic; activity; precisely; recording; changes; fluorescence; yield; produced; series; predetermined; cycles; flashes; respective; sources; computer means; sample chamber; computer controlled; pump sources; computer control; signal conditioning; accurately measure; monitoring means; pump source; fluorometer device; flash source; controlled fluorometer; /250/356/

Citation Formats

Kolber, Zbigniew, and Falkowski, Paul. Computer controlled fluorometer device and method of operating same. United States: N. p., 1990. Web.
Kolber, Zbigniew, & Falkowski, Paul. Computer controlled fluorometer device and method of operating same. United States.
Kolber, Zbigniew, and Falkowski, Paul. 1990. "Computer controlled fluorometer device and method of operating same". United States. doi:. https://www.osti.gov/servlets/purl/867463.
@article{osti_867463,
title = {Computer controlled fluorometer device and method of operating same},
author = {Kolber, Zbigniew and Falkowski, Paul},
abstractNote = {A computer controlled fluorometer device and method of operating same, said device being made to include a pump flash source and a probe flash source and one or more sample chambers in combination with a light condenser lens system and associated filters and reflectors and collimators, as well as signal conditioning and monitoring means and a programmable computer means and a software programmable source of background irradiance that is operable according to the method of the invention to rapidly, efficiently and accurately measure photosynthetic activity by precisely monitoring and recording changes in fluorescence yield produced by a controlled series of predetermined cycles of probe and pump flashes from the respective probe and pump sources that are controlled by the computer means.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = 1990,
month = 1
}

Patent:

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  • A computer controlled fluorometer device and method of operating same, said device being made to include a pump flash source and a probe flash source and one or more sample chambers in combination with a light condenser lens system and associated filters and reflectors and collimators, as well as signal conditioning and monitoring means and a programmable computer means and a software programmable source of background irradiance that is operable according to the method of the invention to rapidly, efficiently and accurately measure photosynthetic activity by precisely monitoring and recording changes in fluorescence yield produced by a controlled series ofmore » predetermined cycles of probe and pump flashes from the respective probe and pump sources that are controlled by the computer means. 13 figs.« less
  • A flexible barrier film has a thickness of from greater than zero to less than 5,000 nanometers and a water vapor transmission rate of no more than 1.times.10.sup.-2 g/m.sup.2/day at 22.degree. C. and 47% relative humidity. The flexible barrier film is formed from a composition, which comprises a multi-functional acrylate. The composition further comprises the reaction product of an alkoxy-functional organometallic compound and an alkoxy-functional organosilicon compound. A method of forming the flexible barrier film includes the steps of disposing the composition on a substrate and curing the composition to form the flexible barrier film. The flexible barrier film maymore » be utilized in organic electronic devices.« less
  • A flexible barrier film has a thickness of from greater than zero to less than 5,000 nanometers and a water vapor transmission rate of no more than 1.times.10.sup.-2 g/m.sup.2/day at 22.degree. C. and 47% relative humidity. The flexible barrier film is formed from a composition, which comprises a multi-functional acrylate. The composition further comprises the reaction product of an alkoxy-functional organometallic compound and an alkoxy-functional organosilicon compound. A method of forming the flexible barrier film includes the steps of disposing the composition on a substrate and curing the composition to form the flexible barrier film. The flexible barrier film maymore » be utilized in organic electronic devices.« less
  • An adhesive flexible barrier film comprises a substrate and a barrier layer disposed on the substrate. The barrier layer is formed from a barrier composition comprising an organosilicon compound. The adhesive flexible barrier film also comprises an adhesive layer disposed on the barrier layer and formed from an adhesive composition. A method of forming the adhesive flexible barrier film comprises the steps of disposing the barrier composition on the substrate to form the barrier layer, disposing the adhesive composition on the barrier layer to form the adhesive layer, and curing the barrier layer and the adhesive layer. The adhesive flexiblemore » barrier film may be utilized in organic electronic devices.« less
  • A microfabricated device and method for electrokinetic transport of a liquid phase biological or chemical material is described. In accordance with one aspect of the present invention there is provided a microchip that is adapted for the simultaneous spatial confinement of electrokinetically driven fluidic material streams on a substrate. The apparatus includes a focusing chamber formed in a surface of the substrate and in fluid communication with two sample fluid channels and three focusing fluid channels. The device further includes electromotive means operatively connected to the sources of the sample fluid and the source of focusing fluid for electrokinetically drivingmore » the respective streams of the sample and focusing fluids through the respective channels into the focusing chamber such that the focusing fluid streams spatially confine the first and second sample fluid streams within the focusing chamber. In accordance with another aspect of this invention, there is provided a cytometry method for analyzing microscopic particles in a fluid medium on a microchip by utilizing the focusing function of the microchip. In the disclosed cytometry process the width of the fluid stream is narrowed in the focusing chamber. The microscopic particles in the focused sample fluid are then detected and/or measured using light scattering or other techniques.« less