The structure of bacteriophage T7 lysozyme, a zinc amidase and an inhibitor of T7 RNA polymerase

Cheng, X; Pflugrath, J W; Zhang, X; Studier, F W

doi:10.1073/pnas.91.9.4034

Title: The structure of bacteriophage T7 lysozyme, a zinc amidase and an inhibitor of T7 RNA polymerase

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Abstract

The lysozyme of bacteriophage T7 is a bifunctional protein that cuts amide bonds in the bacterial cell wall and binds to and inhibits transcription by T7 RNA polymerase. The structure of a mutant T7 lysozyme has been determined by x-ray crystallography and refined at 2.2-{angstrom} resolution. The protein folds into an {alpha}/{beta}-sheet structure that has a prominent cleft. A zinc atom is located in the cleft, bound directly to three amino acids and, through a water molecule, to a fourth. Zinc is required for amidase activity but not for inhibition of T7 RNA polymerase. Alignment of the zinc ligands of T7 lysozyme with those of carboxypeptidase A and thermolysin suggests structural similarity among the catalytic sites for the amidase and these zinc proteases. Mutational analysis identified presumed catalytic residues for amidase activity within the cleft and a surface that appears to be the site of binding to T7 RNA polymerase. Binding of T7 RNA polymerase inhibits amidase activity.

Authors:

Cheng, X; Pflugrath, J W ^[1]; Zhang, X; Studier, F W ^[2]

W.M. Keck Structural Biology Lab., Cold Spring Harbor, NY (United States)
Brookhaven National Lab., Upton, NY (United States)

Publication Date:: Tue Apr 26 00:00:00 EDT 1994

Sponsoring Org.:: USDOE

OSTI Identifier:: 86511

Resource Type:: Journal Article

Journal Name:: Proceedings of the National Academy of Sciences of the United States of America

Additional Journal Information:: Journal Volume: 91; Journal Issue: 9; Other Information: PBD: 26 Apr 1994

Country of Publication:: United States

Language:: English

Subject:: 55 BIOLOGY AND MEDICINE, BASIC STUDIES; LYSOZYME; PROTEIN STRUCTURE; AMIDASES

Citation Formats


                    Cheng, X, Pflugrath, J W, Zhang, X, and Studier, F W. The structure of bacteriophage T7 lysozyme, a zinc amidase and an inhibitor of T7 RNA polymerase.  United States: N. p., 1994. 
        Web.  doi:10.1073/pnas.91.9.4034.

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                    Cheng, X, Pflugrath, J W, Zhang, X, & Studier, F W. The structure of bacteriophage T7 lysozyme, a zinc amidase and an inhibitor of T7 RNA polymerase.  United States.  https://doi.org/10.1073/pnas.91.9.4034

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                    Cheng, X, Pflugrath, J W, Zhang, X, and Studier, F W. 1994.  
        "The structure of bacteriophage T7 lysozyme, a zinc amidase and an inhibitor of T7 RNA polymerase".  United States.  https://doi.org/10.1073/pnas.91.9.4034.

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@article{osti_86511,

  title        = {The structure of bacteriophage T7 lysozyme, a zinc amidase and an inhibitor of T7 RNA polymerase},

  author       = {Cheng, X and Pflugrath, J W and Zhang, X and Studier, F W},

  abstractNote = {The lysozyme of bacteriophage T7 is a bifunctional protein that cuts amide bonds in the bacterial cell wall and binds to and inhibits transcription by T7 RNA polymerase. The structure of a mutant T7 lysozyme has been determined by x-ray crystallography and refined at 2.2-{angstrom} resolution. The protein folds into an {alpha}/{beta}-sheet structure that has a prominent cleft. A zinc atom is located in the cleft, bound directly to three amino acids and, through a water molecule, to a fourth. Zinc is required for amidase activity but not for inhibition of T7 RNA polymerase. Alignment of the zinc ligands of T7 lysozyme with those of carboxypeptidase A and thermolysin suggests structural similarity among the catalytic sites for the amidase and these zinc proteases. Mutational analysis identified presumed catalytic residues for amidase activity within the cleft and a surface that appears to be the site of binding to T7 RNA polymerase. Binding of T7 RNA polymerase inhibits amidase activity.},

  doi          = {10.1073/pnas.91.9.4034},

  url          = {https://www.osti.gov/biblio/86511},
  journal      = {Proceedings of the National Academy of Sciences of the United States of America},
number       = 9,

  volume       = 91,

  place        = {United States},

  year         = {Tue Apr 26 00:00:00 EDT 1994},

  month        = {Tue Apr 26 00:00:00 EDT 1994}

}

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