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Title: Method and apparatus for electrostatically sorting biological cells

Abstract

An improved method of sorting biological cells in a conventional cell sorter apparatus includes generating a fluid jet containing cells to be sorted, measuring the distance between the centers of adjacent droplets in a zone thereof defined at the point where the fluid jet separates into descrete droplets, setting the distance between the center of a droplet in said separation zone and the position along said fluid jet at which the cell is optically sensed for specific characteristics to be an integral multiple of said center-to-center distance, and disabling a charger from electrically charging a specific droplet if a cell is detected by the optical sensor in a position wherein it will be in the neck area between droplets during droplet formation rather than within a predetermined distance from the droplet center.

Inventors:
 [1]
  1. Berkeley, CA
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
864326
Patent Number(s):
US 4347935
Assignee:
United States of America as represented by United States (Washington, DC)
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
method; apparatus; electrostatically; sorting; biological; cells; improved; conventional; cell; sorter; generating; fluid; jet; containing; sorted; measuring; distance; centers; adjacent; droplets; zone; defined; separates; descrete; setting; center; droplet; separation; position; optically; sensed; specific; characteristics; integral; multiple; center-to-center; disabling; charger; electrically; charging; detected; optical; sensor; neck; formation; predetermined; fluid jet; droplet formation; improved method; predetermined distance; biological cells; separation zone; optical sensor; sorting biological; integral multiple; biological cell; jet containing; conventional cell; /209/356/377/

Citation Formats

Merrill, John T. Method and apparatus for electrostatically sorting biological cells. United States: N. p., 1982. Web.
Merrill, John T. Method and apparatus for electrostatically sorting biological cells. United States.
Merrill, John T. 1982. "Method and apparatus for electrostatically sorting biological cells". United States. https://www.osti.gov/servlets/purl/864326.
@article{osti_864326,
title = {Method and apparatus for electrostatically sorting biological cells},
author = {Merrill, John T},
abstractNote = {An improved method of sorting biological cells in a conventional cell sorter apparatus includes generating a fluid jet containing cells to be sorted, measuring the distance between the centers of adjacent droplets in a zone thereof defined at the point where the fluid jet separates into descrete droplets, setting the distance between the center of a droplet in said separation zone and the position along said fluid jet at which the cell is optically sensed for specific characteristics to be an integral multiple of said center-to-center distance, and disabling a charger from electrically charging a specific droplet if a cell is detected by the optical sensor in a position wherein it will be in the neck area between droplets during droplet formation rather than within a predetermined distance from the droplet center.},
doi = {},
url = {https://www.osti.gov/biblio/864326}, journal = {},
number = ,
volume = ,
place = {United States},
year = {1982},
month = {1}
}