Method and apparatus for electrostatically sorting biological cells

Patent · Fri Jan 01 00:00:00 EST 1982

OSTI ID:864326

Merrill, John T ^[1]

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1. Berkeley, CA

An improved method of sorting biological cells in a conventional cell sorter apparatus includes generating a fluid jet containing cells to be sorted, measuring the distance between the centers of adjacent droplets in a zone thereof defined at the point where the fluid jet separates into descrete droplets, setting the distance between the center of a droplet in said separation zone and the position along said fluid jet at which the cell is optically sensed for specific characteristics to be an integral multiple of said center-to-center distance, and disabling a charger from electrically charging a specific droplet if a cell is detected by the optical sensor in a position wherein it will be in the neck area between droplets during droplet formation rather than within a predetermined distance from the droplet center.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
• DOE Contract Number: W-7405-ENG-48
• Assignee: United States of America as represented by United States (Washington, DC)
• Patent Number(s): US 4347935
• OSTI ID: 864326
• Country of Publication: United States
• Language: English

Related Subjects

method
apparatus
electrostatically
sorting
biological
cells
improved
conventional
cell
sorter
generating
fluid
jet
containing
sorted
measuring
distance
centers
adjacent
droplets
zone
defined
separates
descrete
setting
center
droplet
separation
position
optically
sensed
specific
characteristics
integral
multiple
center-to-center
disabling
charger
electrically
charging
detected
optical
sensor
neck
formation
predetermined
fluid jet
droplet formation
improved method
predetermined distance
biological cells
separation zone
optical sensor
sorting biological
integral multiple
biological cell
jet containing
conventional cell
/209/356/377/