Apparatus for electrophoresis separation

Patent · 31 December 1977

OSTI ID:863079

Anderson, Norman L ^[1]

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1. Willowbrook, IL

An apparatus is disclosed for simultaneously performing electrophoresis separations on a plurality of slab gels containing samples of protein, protein subunits or nucleic acids. A reservoir of buffer solution is divided into three compartments by two parallel partitions having vertical slots spaced along their length. A sheet of flexible, electrically insulative material is attached to each partition and is provided with vertical slits aligned with the slots. Slab-gel holders are received within the slots with the flexible material folded outwardly as flaps from the slits to overlay portions of the holder surfaces and thereby act as electrical and liquid seals. An elongated, spaghetti-like gel containing a sample of specimen that was previously separated by isoelectric focusing techniques is vertically positioned along a marginal edge portion of the slab gel. On application of an electrical potential between the two outer chambers of buffer solution, a second dimensional electrophoresis separation in accordance with molecular weight occurs as the specimen molecules migrate across the slab gel.

• Research Organization: Argonne National Laboratory (ANL), Argonne, IL
• Assignee: United States of America as represented by United States (Washington, DC)
• Patent Number(s): US 4088561
• OSTI ID: 863079
• Country of Publication: United States
• Language: English

Related Subjects

/204/436/
accordance
acids
aligned
apparatus
application
attached
buffer
buffer solution
chambers
compartments
containing
containing sample
dimensional
disclosed
divided
edge
edge portion
electrical
electrical potential
electrically
electrically insulative
electrophoresis
electrophoresis separation
electrophoresis separations
elongated
flaps
flexible
flexible material
focusing
folded
gels
holder
holders
insulative
insulative material
isoelectric
length
liquid
liquid seal
marginal
material
migrate
molecular
molecular weight
molecules
nucleic
nucleic acid
nucleic acids
occurs
outer
outwardly
overlay
parallel
partition
partitions
performing
plurality
portion
portions
positioned
potential
previously
protein
provided
received
reservoir
sample
samples
seals
separated
separation
separations
sheet
simultaneously
slab
slab-gel
slab-gel holder
slab-gel holders
slits
slots
solution
spaced
spaghetti-like
specimen
subunits
surfaces
techniques
vertical
vertical slit
vertical slot
vertically
vertically position
weight