skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: A molecular ruler based on plasmon coupling of single gold andsilver nanoparticles

Abstract

Molecular rulers based on Foerster Resonance Energy Transfer (FRET) that report conformational changes and intramolecular distances of single biomolecules have helped to understand important biological processes. However, these rulers suffer from low and fluctuating signal intensities from single dyes and limited observation time due to photobleaching. The plasmon resonance in noble metal particles has been suggested as an alternative probe to overcome the limitations of organic fluorophores and the coupling of plasmons in nearby particles has been exploited to detect particle aggregation by a distinct color change in bulk experiments. Here we demonstrate that plasmon coupling can be used to monitor distances between single pairs of gold and silver nanoparticles. We use this effect to follow the directed assembly of gold and silver nanoparticle dimers in real time and to study the time dynamics of single DNA hybridization events. These ''plasmon rulers'' allowed us to continuously monitor separations of up to 70 nm for more than 3000 seconds. Single molecule in vitro studies of biological processes previously inaccessible with fluorescence based molecular rulers are enabled with plasmon rulers with extended time and distance range.

Authors:
; ; ;
Publication Date:
Research Org.:
Ernest Orlando Lawrence Berkeley NationalLaboratory, Berkeley, CA (US)
Sponsoring Org.:
USDOE Director. Office of Science. Office of Basic EnergySciences, Los Alamos National Laboratory Contract W-7405-ENG-36; Alexander von Humboldt Foundation, Otto A Wipprecht Foundation, DeutscheForschungsgemeinschaft
OSTI Identifier:
862316
Report Number(s):
LBID-2572
Journal ID: ISSN 1087-0156; NABIF9; R&D Project: 516070; BnR: KC0203010; TRN: US0600648
DOE Contract Number:  
DE-AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
Nature Biotechnology
Additional Journal Information:
Journal Volume: 23; Journal Issue: 6; Related Information: Journal Publication Date: 06/2005; Journal ID: ISSN 1087-0156
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS; COLOR; CONFORMATIONAL CHANGES; DIMERS; DNA HYBRIDIZATION; DYES; ENERGY TRANSFER; FLUORESCENCE; GOLD; IN VITRO; MONITORS; PLASMONS; PROBES; RESONANCE; SILVER

Citation Formats

Sonnichsen, Carsten, Reinhard, Bjorn M, Liphardt, Jan, and Alivisatos, A Paul. A molecular ruler based on plasmon coupling of single gold andsilver nanoparticles. United States: N. p., 2005. Web. doi:10.1038/nbt1100.
Sonnichsen, Carsten, Reinhard, Bjorn M, Liphardt, Jan, & Alivisatos, A Paul. A molecular ruler based on plasmon coupling of single gold andsilver nanoparticles. United States. doi:10.1038/nbt1100.
Sonnichsen, Carsten, Reinhard, Bjorn M, Liphardt, Jan, and Alivisatos, A Paul. Sun . "A molecular ruler based on plasmon coupling of single gold andsilver nanoparticles". United States. doi:10.1038/nbt1100. https://www.osti.gov/servlets/purl/862316.
@article{osti_862316,
title = {A molecular ruler based on plasmon coupling of single gold andsilver nanoparticles},
author = {Sonnichsen, Carsten and Reinhard, Bjorn M and Liphardt, Jan and Alivisatos, A Paul},
abstractNote = {Molecular rulers based on Foerster Resonance Energy Transfer (FRET) that report conformational changes and intramolecular distances of single biomolecules have helped to understand important biological processes. However, these rulers suffer from low and fluctuating signal intensities from single dyes and limited observation time due to photobleaching. The plasmon resonance in noble metal particles has been suggested as an alternative probe to overcome the limitations of organic fluorophores and the coupling of plasmons in nearby particles has been exploited to detect particle aggregation by a distinct color change in bulk experiments. Here we demonstrate that plasmon coupling can be used to monitor distances between single pairs of gold and silver nanoparticles. We use this effect to follow the directed assembly of gold and silver nanoparticle dimers in real time and to study the time dynamics of single DNA hybridization events. These ''plasmon rulers'' allowed us to continuously monitor separations of up to 70 nm for more than 3000 seconds. Single molecule in vitro studies of biological processes previously inaccessible with fluorescence based molecular rulers are enabled with plasmon rulers with extended time and distance range.},
doi = {10.1038/nbt1100},
journal = {Nature Biotechnology},
issn = {1087-0156},
number = 6,
volume = 23,
place = {United States},
year = {2005},
month = {5}
}