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Title: Sequencing and comparing whole mitochondrial genomes ofanimals

Abstract

Comparing complete animal mitochondrial genome sequences is becoming increasingly common for phylogenetic reconstruction and as a model for genome evolution. Not only are they much more informative than shorter sequences of individual genes for inferring evolutionary relatedness, but these data also provide sets of genome-level characters, such as the relative arrangements of genes, that can be especially powerful. We describe here the protocols commonly used for physically isolating mtDNA, for amplifying these by PCR or RCA, for cloning,sequencing, assembly, validation, and gene annotation, and for comparing both sequences and gene arrangements. On several topics, we offer general observations based on our experiences to date with determining and comparing complete mtDNA sequences.

Authors:
; ;
Publication Date:
Research Org.:
Ernest Orlando Lawrence Berkeley NationalLaboratory, Berkeley, CA (US)
Sponsoring Org.:
USDOE Director. Office of Science. Biological andEnvironmental Research; National Science Foundation Grants DEB-0089624and EAR-0120646 and DEB-9807100
OSTI Identifier:
861135
Report Number(s):
LBNL-55278
R&D Project: 626849; BnR: KP1103010; TRN: US200601%%637
DOE Contract Number:  
DE-AC02-05CH11231
Resource Type:
Book
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ANIMALS; GENES; VALIDATION; mitochondrial genome DNA phylogenetics

Citation Formats

Boore, Jeffrey L., Macey, J. Robert, and Medina, Monica. Sequencing and comparing whole mitochondrial genomes ofanimals. United States: N. p., 2005. Web.
Boore, Jeffrey L., Macey, J. Robert, & Medina, Monica. Sequencing and comparing whole mitochondrial genomes ofanimals. United States.
Boore, Jeffrey L., Macey, J. Robert, and Medina, Monica. Fri . "Sequencing and comparing whole mitochondrial genomes ofanimals". United States. https://www.osti.gov/servlets/purl/861135.
@article{osti_861135,
title = {Sequencing and comparing whole mitochondrial genomes ofanimals},
author = {Boore, Jeffrey L. and Macey, J. Robert and Medina, Monica},
abstractNote = {Comparing complete animal mitochondrial genome sequences is becoming increasingly common for phylogenetic reconstruction and as a model for genome evolution. Not only are they much more informative than shorter sequences of individual genes for inferring evolutionary relatedness, but these data also provide sets of genome-level characters, such as the relative arrangements of genes, that can be especially powerful. We describe here the protocols commonly used for physically isolating mtDNA, for amplifying these by PCR or RCA, for cloning,sequencing, assembly, validation, and gene annotation, and for comparing both sequences and gene arrangements. On several topics, we offer general observations based on our experiences to date with determining and comparing complete mtDNA sequences.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2005},
month = {4}
}

Book:
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