Gene expression profiles of Nitrosomonas europaea, an obligate chemolitotroph
Nitrosomonas europaea is an aerobic lithoautotrophic bacterium that uses ammonia (NH3) as its energy source. As a nitrifier, it is an important participant in the nitrogen cycle, which can also influence the carbon cycle. The focus of this work was to explore the genetic structure and mechanisms underlying the lithoautotrophic growth style of N. europaea. Whole genome gene expression. The gene expression profile of cells in exponential growth and during starvation was analyzed using microarrays. During growth, 98% of the genes increased in expression at least two fold compared to starvation conditions. In growing cells, approximately 30% of the genes were expressed eight fold higher, Approximately 10% were expressed more than 15 fold higher. Approximately 3% (91 genes) were expressed to more than 20 fold of their levels in starved cells. Carbon fixation gene expression. N. europaea fixes carbon via the Calvin-Benson-Bassham (CBB) cycle via a type I ribulose bisphosphate carboxylase/oxygenase (RubisCO). This study showed that transcription of cbb genes was up-regulated when the carbon source was limited, while amo, hao and other energy harvesting related genes were down-regulated. Iron related gene expression. Because N. europaea has a relatively high content of hemes, sufficient Fe must be available in the medium for it to grow. The genome revealed that approximately 5% of the coding genes in N. europaea are dedicated to Fe transport and assimilation. Nonetheless, with the exception of citrate biosynthesis genes, N. europaea lacks genes for siderophore production. The Fe requirements for growth and the expression of the putative membrane siderophore receptors were determined. The N. europaea genome has over 100 putative genes ({approx}5% of the coding genes) related to Fe uptake and its siderophore receptors could be grouped phylogenetically in four clusters. Fe related genes, such as a number of TonB-dependent Fe-siderophore receptors for ferrichrome and enterobactin, and diffusion protein OmpC were expressed to higher levels under Fe limitation. N. europaea has a high Fe requirement and under Fe limiting conditions (0.2 ?M), is capable to assimilate up to 70% of the available Fe without the ability to produce siderophores.
- Research Organization:
- Oregon State University. Corvallis OR 97331
- Sponsoring Organization:
- USDOE Office of Science (SC); Office of Acquisition and Assistance; Office of Biological and Energy Research
- DOE Contract Number:
- FG03-01ER63149
- OSTI ID:
- 840845
- Report Number(s):
- DOE/ER/63149; G0057A; TRN: US200707%%137
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
54 ENVIRONMENTAL SCIENCES
60 APPLIED LIFE SCIENCES
AMMONIA
BIOSYNTHESIS
CARBON
CARBON CYCLE
CARBON SOURCES
CITRATES
DIFFUSION
ENERGY SOURCES
FASTING
GENES
GENETICS
HARVESTING
IRON
MEMBRANES
NITROGEN CYCLE
PROTEINS
RIBULOSE
TRANSCRIPTION
Nitrosomonas europaea
lithotrophy
gene expression