Effects of Cr(VI) long-term and low-dose action on mammalian antioxidant enzymes (an in vitro study)
In order to investigate the low-dose long-term Cr(VI) action on antioxidant enzymes in cultured mammalian cells we estimated the activity of glutathione dependent antioxidant enzymes, catalase and superoxide dismutase (SOD) under various chromium concentrations in human epithelial-like L-41 cells. The long-term action of 20 mu-M causes the toxicity that results in losing of the cell viability by activating the apoptotic process, as identified by morphological analysis, the activation of caspase-3, and DNA fragmentation. The toxic chromium concentration totally destroys glutathione antioxidant system, and diminishes the activity of catalase and cytosolic Cu, ZnSOD. The non-toxic concentration (2 mu-M) causes the activation of the antioxidant defense systems, and they neutralize the oxidative impact.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- US Department of Energy (US)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 834905
- Report Number(s):
- LBNL-55999; JIBIDJ; TRN: US200433%%457
- Journal Information:
- Journal of Inorganic Biochemistry, Vol. 98; Other Information: Journal Publication Date: 03/2004; PBD: 1 Aug 2003; ISSN 0162-0134
- Country of Publication:
- United States
- Language:
- English
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