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Title: The spectrum of mutation produced by low dose radiation

Abstract

Inherited mutations are the basis of evolution and acquired mutations in humans are important in ageing, cancer and possibly various forms of tissue degeneration. Mutations are responsible for many of the long-term effects of radiation. However, sensitive direct detection of mutations in humans has been difficult. The aims of the project were to develop methods for the sensitive enumeration of mutations in DNA, to measure mutation frequencies in a wide variety of tissue types and to quantify the mutational effect of direct oxidative damage produced by radiation, at both high and low doses. The project was successful in developing a sensitive method which could detect mutations directly in the genetic material, DNA at a sensitivity of 1 mutated molecule in 1000000000 unmutated molecules. However a number of methodological problems had to be overcome and lack of ongoing funding made it impossible to fulfill all of the aims of the project

Authors:
;
Publication Date:
Research Org.:
Flinders University, Adelaide, SA (AU)
Sponsoring Org.:
USDOE Office of Environmental Management (EM); USDOE Office of Science (SC) (US)
OSTI Identifier:
834002
Report Number(s):
DOE/ER/62906- Final Report
TRN: US0500455
DOE Contract Number:  
FG02-00ER62906
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: 31 Oct 2004
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; 61 RADIATION PROTECTION AND DOSIMETRY; DETECTION; DNA; GENETICS; MUTATIONS; NEOPLASMS; RADIATIONS; SENSITIVITY; MUTATION; PCR; LOW-DOSE RADIATION

Citation Formats

Morley,Alexander,A, and Turner, David,R. The spectrum of mutation produced by low dose radiation. United States: N. p., 2004. Web. doi:10.2172/834002.
Morley,Alexander,A, & Turner, David,R. The spectrum of mutation produced by low dose radiation. United States. doi:10.2172/834002.
Morley,Alexander,A, and Turner, David,R. Sun . "The spectrum of mutation produced by low dose radiation". United States. doi:10.2172/834002. https://www.osti.gov/servlets/purl/834002.
@article{osti_834002,
title = {The spectrum of mutation produced by low dose radiation},
author = {Morley,Alexander,A and Turner, David,R},
abstractNote = {Inherited mutations are the basis of evolution and acquired mutations in humans are important in ageing, cancer and possibly various forms of tissue degeneration. Mutations are responsible for many of the long-term effects of radiation. However, sensitive direct detection of mutations in humans has been difficult. The aims of the project were to develop methods for the sensitive enumeration of mutations in DNA, to measure mutation frequencies in a wide variety of tissue types and to quantify the mutational effect of direct oxidative damage produced by radiation, at both high and low doses. The project was successful in developing a sensitive method which could detect mutations directly in the genetic material, DNA at a sensitivity of 1 mutated molecule in 1000000000 unmutated molecules. However a number of methodological problems had to be overcome and lack of ongoing funding made it impossible to fulfill all of the aims of the project},
doi = {10.2172/834002},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {10}
}