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Title: Novel Mass Spectrometry Mutation Screening for Contaminant Impact Analysis

Abstract

The objective of this program is to develop innovative mass spectrometry technology to achieve fast mutation analysis and to reveal the linkage between gene mutation and contaminants. Mass spectrometry has the potential to achieve very fast speed sample analysis. However, the poor mass resolution and low detection efficiency for long DNAs limit the broad application for mutation analysis. New innovative approaches for improving mass resolution and detection sensitivity needs to be pursued to help to achieve rapid DNA screening. Allele specific polymerase chain reaction (ASPCR) coupled with mass spectrometry can be very valuable for DNA mutation detection. This technology is expected to be applied to wildlife species such as fish for the genotoxic effect of hazardous waste to be assessed at DNA level.

Authors:
;
Publication Date:
Research Org.:
Oak Ridge National Lab., Oak Ridge, TN (US)
Sponsoring Org.:
USDOE Office of Environmental Management (EM) (US)
OSTI Identifier:
829892
Report Number(s):
EMSP-60218-2000
R&D Project: EMSP 60218; TRN: US200429%%65
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: 1 Jun 2000
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; DETECTION; DNA; EFFICIENCY; GENE MUTATIONS; MASS RESOLUTION; MASS SPECTROSCOPY; MUTATIONS; POLYMERASE CHAIN REACTION; SENSITIVITY; VELOCITY; WASTES

Citation Formats

Cehn, Winston Chumg-Hsuan, and Lee, Kai-Lin. Novel Mass Spectrometry Mutation Screening for Contaminant Impact Analysis. United States: N. p., 2000. Web. doi:10.2172/829892.
Cehn, Winston Chumg-Hsuan, & Lee, Kai-Lin. Novel Mass Spectrometry Mutation Screening for Contaminant Impact Analysis. United States. doi:10.2172/829892.
Cehn, Winston Chumg-Hsuan, and Lee, Kai-Lin. Thu . "Novel Mass Spectrometry Mutation Screening for Contaminant Impact Analysis". United States. doi:10.2172/829892. https://www.osti.gov/servlets/purl/829892.
@article{osti_829892,
title = {Novel Mass Spectrometry Mutation Screening for Contaminant Impact Analysis},
author = {Cehn, Winston Chumg-Hsuan and Lee, Kai-Lin},
abstractNote = {The objective of this program is to develop innovative mass spectrometry technology to achieve fast mutation analysis and to reveal the linkage between gene mutation and contaminants. Mass spectrometry has the potential to achieve very fast speed sample analysis. However, the poor mass resolution and low detection efficiency for long DNAs limit the broad application for mutation analysis. New innovative approaches for improving mass resolution and detection sensitivity needs to be pursued to help to achieve rapid DNA screening. Allele specific polymerase chain reaction (ASPCR) coupled with mass spectrometry can be very valuable for DNA mutation detection. This technology is expected to be applied to wildlife species such as fish for the genotoxic effect of hazardous waste to be assessed at DNA level.},
doi = {10.2172/829892},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {6}
}

Technical Report:

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