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Title: Molecular Profiling of Microbial Communities from Contaminated Sources: Use of Subtractive Cloning Methods and rDNA Spacer Sequences

Abstract

The major objective of the research was to provide appropriate sequences and to assemble a DNA arrays of oligonucleotides to be used for rapid profiling of microbial populations, from polluted areas and from areas of other interest. The sequences to be assigned to the DNA array are chosen from cloned genomic DNA from groundwater at DOE sites containing organic solvents. The sites, Hanford Nuclear Plant and Lawrence Livermore Site 300 (LLNL), have well characterized pollutant histories, which have been provided by our collaborators.

Authors:
Publication Date:
Research Org.:
University of Maryland at Baltimore, Baltimore, Maryland (US)
Sponsoring Org.:
USDOE Office of Environmental Management (EM) (US)
OSTI Identifier:
827425
Report Number(s):
EMSP-55152-2000
R&D Project: EMSP 55152; TRN: US200425%%662
DOE Contract Number:  
FG07-96ER62320
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: 1 Jun 2000
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; CLONING; COMMUNITIES; DNA; LAWRENCE LIVERMORE NATIONAL LABORATORY; OLIGONUCLEOTIDES; ORGANIC SOLVENTS; POLLUTANTS; SPACERS

Citation Formats

Robb, Frank T. Molecular Profiling of Microbial Communities from Contaminated Sources: Use of Subtractive Cloning Methods and rDNA Spacer Sequences. United States: N. p., 2000. Web. doi:10.2172/827425.
Robb, Frank T. Molecular Profiling of Microbial Communities from Contaminated Sources: Use of Subtractive Cloning Methods and rDNA Spacer Sequences. United States. doi:10.2172/827425.
Robb, Frank T. Thu . "Molecular Profiling of Microbial Communities from Contaminated Sources: Use of Subtractive Cloning Methods and rDNA Spacer Sequences". United States. doi:10.2172/827425. https://www.osti.gov/servlets/purl/827425.
@article{osti_827425,
title = {Molecular Profiling of Microbial Communities from Contaminated Sources: Use of Subtractive Cloning Methods and rDNA Spacer Sequences},
author = {Robb, Frank T},
abstractNote = {The major objective of the research was to provide appropriate sequences and to assemble a DNA arrays of oligonucleotides to be used for rapid profiling of microbial populations, from polluted areas and from areas of other interest. The sequences to be assigned to the DNA array are chosen from cloned genomic DNA from groundwater at DOE sites containing organic solvents. The sites, Hanford Nuclear Plant and Lawrence Livermore Site 300 (LLNL), have well characterized pollutant histories, which have been provided by our collaborators.},
doi = {10.2172/827425},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {6}
}

Technical Report:

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