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Title: Monitoring Genetic and Metabolic Potential for In-Situ Bioremediation: Mass Spectrometry

Abstract

A number of DOE sites are contaminated with dense non-aqueous phase liquids (DNAPLs) such as carbon tetrachloride and trichloroethylene. At many of these sites, microbial bioremediation is an attractive strategy for cleanup, since it has the potential to degrade DNAPLs in situ. A rapid screening method to determine the broad range potential of a site's microbial population for contaminant degradation would greatly facilitate assessment for in situ bioremediation, as well as for monitoring ongoing bioremediation treatment. Current laboratory-based treatability methods are cumbersome and expensive. In this project, we are developing methods based on matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for rapid and accurate detection of polymerase chain reaction (PCR) products from microbial genes involved in biodegradation of pollutants. PCR primers are being developed to amplify DNA sequences that are amenable to MALDI-MS detection. This work will lay the foundation for development of a field-portable MS-based technique for rapid on site assessment and monitoring of bioremediation processes.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Oak Ridge National Lab., TN; University of Washington, Seattle, WA (US)
Sponsoring Org.:
USDOE Office of Environmental Management (EM) (US)
OSTI Identifier:
827403
Report Number(s):
EMSP-55108-2000
R&D Project: EMSP 55108; TRN: US200425%%643
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: 1 Jun 2000
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; BIODEGRADATION; BIOREMEDIATION; CARBON TETRACHLORIDE; DETECTION; DNA; GENES; GENETICS; LASERS; MASS SPECTROSCOPY; MONITORING; POLLUTANTS; POLYMERASE CHAIN REACTION

Citation Formats

Buchanan, Michelle V, Britt, Phillip F, Doktycz, Mitchel J, Hurst, Gregory B, and Lidstrom, Mary E. Monitoring Genetic and Metabolic Potential for In-Situ Bioremediation: Mass Spectrometry. United States: N. p., 2000. Web. doi:10.2172/827403.
Buchanan, Michelle V, Britt, Phillip F, Doktycz, Mitchel J, Hurst, Gregory B, & Lidstrom, Mary E. Monitoring Genetic and Metabolic Potential for In-Situ Bioremediation: Mass Spectrometry. United States. doi:10.2172/827403.
Buchanan, Michelle V, Britt, Phillip F, Doktycz, Mitchel J, Hurst, Gregory B, and Lidstrom, Mary E. Thu . "Monitoring Genetic and Metabolic Potential for In-Situ Bioremediation: Mass Spectrometry". United States. doi:10.2172/827403. https://www.osti.gov/servlets/purl/827403.
@article{osti_827403,
title = {Monitoring Genetic and Metabolic Potential for In-Situ Bioremediation: Mass Spectrometry},
author = {Buchanan, Michelle V and Britt, Phillip F and Doktycz, Mitchel J and Hurst, Gregory B and Lidstrom, Mary E},
abstractNote = {A number of DOE sites are contaminated with dense non-aqueous phase liquids (DNAPLs) such as carbon tetrachloride and trichloroethylene. At many of these sites, microbial bioremediation is an attractive strategy for cleanup, since it has the potential to degrade DNAPLs in situ. A rapid screening method to determine the broad range potential of a site's microbial population for contaminant degradation would greatly facilitate assessment for in situ bioremediation, as well as for monitoring ongoing bioremediation treatment. Current laboratory-based treatability methods are cumbersome and expensive. In this project, we are developing methods based on matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for rapid and accurate detection of polymerase chain reaction (PCR) products from microbial genes involved in biodegradation of pollutants. PCR primers are being developed to amplify DNA sequences that are amenable to MALDI-MS detection. This work will lay the foundation for development of a field-portable MS-based technique for rapid on site assessment and monitoring of bioremediation processes.},
doi = {10.2172/827403},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {6}
}

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