Imaging Whole Escherichia Coli Bacteria by Using Single-Particle X-Ray Diffraction
Abstract
We report the first experimental recording, to our knowledge, of the diffraction pattern from intact Escherichia coli bacteria using coherent x-rays with a wavelength of 2 {angstrom}. By using the over-sampling phasing method, a real space image at a resolution of 30 nm was directly reconstructed from the diffraction pattern. An R factor used for characterizing the quality of the reconstruction was in the range of 5%, which demonstrated the reliability of the reconstruction process. The distribution of proteins inside the bacteria labeled with manganese oxide has been identified and this distribution confirmed by fluorescence microscopy images. Compared with lens-based microscopy, this diffraction-based imaging approach can examine thicker samples, such as whole cultured cells, in three dimensions with resolution limited only by radiation damage. Looking forward, the successful recording and reconstruction of diffraction patterns from biological samples reported here represent an important step toward the potential of imaging single biomolecules at near-atomic resolution by combining single-particle diffraction with x-ray free electron lasers.
- Authors:
- Publication Date:
- Research Org.:
- Stanford Linear Accelerator Center, Menlo Park, CA (US)
- Sponsoring Org.:
- USDOE Office of Science (US)
- OSTI Identifier:
- 826962
- Report Number(s):
- SLAC-PUB-10482
TRN: US200428%%1550
- DOE Contract Number:
- AC03-76SF00515
- Resource Type:
- Technical Report
- Resource Relation:
- Other Information: PBD: 4 Jun 2004
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATION; BACTERIA; DIFFRACTION; DIMENSIONS; ESCHERICHIA COLI; FLUORESCENCE; FREE ELECTRON LASERS; MANGANESE OXIDES; MICROSCOPY; PROTEINS; R FACTORS; RADIATIONS; RELIABILITY; RESOLUTION; WAVELENGTHS; X-RAY DIFFRACTION
Citation Formats
Miao, J. Imaging Whole Escherichia Coli Bacteria by Using Single-Particle X-Ray Diffraction. United States: N. p., 2004.
Web. doi:10.2172/826962.
Miao, J. Imaging Whole Escherichia Coli Bacteria by Using Single-Particle X-Ray Diffraction. United States. doi:10.2172/826962.
Miao, J. Fri .
"Imaging Whole Escherichia Coli Bacteria by Using Single-Particle X-Ray Diffraction". United States.
doi:10.2172/826962. https://www.osti.gov/servlets/purl/826962.
@article{osti_826962,
title = {Imaging Whole Escherichia Coli Bacteria by Using Single-Particle X-Ray Diffraction},
author = {Miao, J.},
abstractNote = {We report the first experimental recording, to our knowledge, of the diffraction pattern from intact Escherichia coli bacteria using coherent x-rays with a wavelength of 2 {angstrom}. By using the over-sampling phasing method, a real space image at a resolution of 30 nm was directly reconstructed from the diffraction pattern. An R factor used for characterizing the quality of the reconstruction was in the range of 5%, which demonstrated the reliability of the reconstruction process. The distribution of proteins inside the bacteria labeled with manganese oxide has been identified and this distribution confirmed by fluorescence microscopy images. Compared with lens-based microscopy, this diffraction-based imaging approach can examine thicker samples, such as whole cultured cells, in three dimensions with resolution limited only by radiation damage. Looking forward, the successful recording and reconstruction of diffraction patterns from biological samples reported here represent an important step toward the potential of imaging single biomolecules at near-atomic resolution by combining single-particle diffraction with x-ray free electron lasers.},
doi = {10.2172/826962},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jun 04 00:00:00 EDT 2004},
month = {Fri Jun 04 00:00:00 EDT 2004}
}
-
Investigation Using X-ray Diffraction of the Formation of the $gamma$' Phase in U-Mo Alloys; ETUDE PAR DIFFRACTION DES RAYONS X DE LA FORMATION DE LA PHASE $gamma$' DANS LES ALLIAGES U-Mo
The decomposition of the gamma phase of uranium-molybdenum alloys does not take place in the classical manner. The equilibrium products are not formed immediately after the start of the decomposition. The alpha phase is first precipitated and this liberates molybdenum which enriches the gamma phase. When this gamma phase is suficiently enriched, it arranges itself so that the equilibrium product gamma ' appears. (auth)