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Title: Microbial Flocculant for Nature Soda

Abstract

Microbial flocculant for nature soda has been studied. Lactobacillus TRJ21, which was able to produce an excellent biopolymer flocculant for nature soda, was obtained in our lab. The microbial flocculant was mainly produced when the bacteria laid in stationary growth phase. Fructose or glucose, as carbon sources, were more favorable for the bacterial growth and flocculant production. The bacteria was able to use ammonium sulfate or Urea as nitrogen to produce flocculant, but was not able to use peptone effectively. High C/N ratio was more favorable to Lactobacillus TRJ21 growth and flocculant production than low C/N ratio. The biopolymer flocculant was mainly composed of polysaccharide and protein with a molecular weight 1.38x106 by gel permeation chromatography. It was able to be easily purified from the culture medium by acetone. Protein in the flocculant was tested for the flocculating activity ingredient by heating the flocculant.

Authors:
; ;
Publication Date:
Research Org.:
Tsinghua University, Beijing (CN); Inner Mongolia Polytechnic University, Hohhot (CN)
Sponsoring Org.:
Sponsor not identified (US)
OSTI Identifier:
826124
Resource Type:
Conference
Resource Relation:
Conference: 227th American Chemical Society Meeting, Anaheim, CA (US), 03/28/2004--04/01/2004; Other Information: PBD: 31 Mar 2004
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; ACETONE; AMMONIUM SULFATES; BACTERIA; CARBON SOURCES; FRUCTOSE; GEL PERMEATION CHROMATOGRAPHY; GLUCOSE; HEATING; LACTOBACILLUS; MOLECULAR WEIGHT; NITROGEN; PEPTONE; POLYSACCHARIDES; PROTEINS; UREA

Citation Formats

Qin, Peiyong, Zhang, Tong, and Chen, Cuixian. Microbial Flocculant for Nature Soda. United States: N. p., 2004. Web.
Qin, Peiyong, Zhang, Tong, & Chen, Cuixian. Microbial Flocculant for Nature Soda. United States.
Qin, Peiyong, Zhang, Tong, and Chen, Cuixian. Wed . "Microbial Flocculant for Nature Soda". United States. https://www.osti.gov/servlets/purl/826124.
@article{osti_826124,
title = {Microbial Flocculant for Nature Soda},
author = {Qin, Peiyong and Zhang, Tong and Chen, Cuixian},
abstractNote = {Microbial flocculant for nature soda has been studied. Lactobacillus TRJ21, which was able to produce an excellent biopolymer flocculant for nature soda, was obtained in our lab. The microbial flocculant was mainly produced when the bacteria laid in stationary growth phase. Fructose or glucose, as carbon sources, were more favorable for the bacterial growth and flocculant production. The bacteria was able to use ammonium sulfate or Urea as nitrogen to produce flocculant, but was not able to use peptone effectively. High C/N ratio was more favorable to Lactobacillus TRJ21 growth and flocculant production than low C/N ratio. The biopolymer flocculant was mainly composed of polysaccharide and protein with a molecular weight 1.38x106 by gel permeation chromatography. It was able to be easily purified from the culture medium by acetone. Protein in the flocculant was tested for the flocculating activity ingredient by heating the flocculant.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {3}
}

Conference:
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