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Title: Development of a Charged Particle Microbeam for Targeted and Single Particle Subcellular Irradiation

Abstract

The development of a charged particle microbeam for single particle, subcellular irradiations at the Massachusetts Institute of Technology Laboratory for Accelerator Beam Applications (MIT LABA) was initiated under this NEER aeard. The Microbeam apparatus makes use of a pre-existing electrostatic accelerator with a horizontal beam tube.

Authors:
Publication Date:
Research Org.:
Massachusetts Institute of Technology (US)
Sponsoring Org.:
(US)
OSTI Identifier:
822003
Report Number(s):
DOE/ID13643
TRN: US0401328
DOE Contract Number:
FG07-98ID13643
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: 12 Mar 2004
Country of Publication:
United States
Language:
English
Subject:
43 PARTICLE ACCELERATORS; ACCELERATORS; CHARGED PARTICLES; ELECTROSTATIC ACCELERATORS; IRRADIATION; MASSACHUSETTS; RADIATION EFFECTS; ALPHA PARTICLES; CHARGED PARTICLES, MICROBEAM, RADIATION EFFECTS, PROTONS, ALPHA PARTICLES

Citation Formats

Yanch, Jacquelyn C. Development of a Charged Particle Microbeam for Targeted and Single Particle Subcellular Irradiation. United States: N. p., 2004. Web. doi:10.2172/822003.
Yanch, Jacquelyn C. Development of a Charged Particle Microbeam for Targeted and Single Particle Subcellular Irradiation. United States. doi:10.2172/822003.
Yanch, Jacquelyn C. 2004. "Development of a Charged Particle Microbeam for Targeted and Single Particle Subcellular Irradiation". United States. doi:10.2172/822003. https://www.osti.gov/servlets/purl/822003.
@article{osti_822003,
title = {Development of a Charged Particle Microbeam for Targeted and Single Particle Subcellular Irradiation},
author = {Yanch, Jacquelyn C.},
abstractNote = {The development of a charged particle microbeam for single particle, subcellular irradiations at the Massachusetts Institute of Technology Laboratory for Accelerator Beam Applications (MIT LABA) was initiated under this NEER aeard. The Microbeam apparatus makes use of a pre-existing electrostatic accelerator with a horizontal beam tube.},
doi = {10.2172/822003},
journal = {},
number = ,
volume = ,
place = {United States},
year = 2004,
month = 3
}

Technical Report:

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  • An irradiation facility, able to expose cellular and subcellular targets to a precise number of particles, has been developed at CENBG for applications in radiobiology. The development of this facility was based on an existing horizontal focused microbeam developed in the early 90's for material analysis. The focusing properties of the line allow the delivering of proton or alpha particle beams in the 1-3.5 MeV energy range with a spatial resolution down to about 1 {mu}m under vacuum. For irradiation of living cells, a removable stage has been developed to extract the beam into air while preserving the analytical capabilitiesmore » of the microbeam line under vacuum. This stage includes a high resolution epifluorescence microscope for online visualization of the cells and a motorized stage for cell positioning. Single particle control is ensured by a fast electrostatic deflector triggered by the signal induced by the particles through a transmission detector just before reaching the target. A dedicated software, based on an object-oriented architecture, has been designed to control the entire experiment. This includes semiautomatic calibration procedures (necessary to achieve the micron precision) and semiautomatic irradiation procedures used for targeting a large number of individual cells. In air irradiation of solid track detectors has permitted us to estimate that 99.5% of the particles are delivered on the target at a distance lower than 5 {mu}m from the beam center when an alpha particles beam is used. The targeting precision of the overall irradiation procedure, which reflects the alignment precision of the beam center with the target center, has been estimated to be within {+-}2 {mu}m. First experiments involving cells in culture have permitted to estimate an irradiation rate of 2000 cells per hour. This article presents the overall experimental facility and the tests performed for its validation for the irradiation of individual cells in their culture medium.« less
  • The single particle orbit code, TIBRO, has been modified extensively to improve the interpolation methods used and to allow use of vector potential fields in the simulation of charged particle orbits on a 3D domain. A 3D cubic B-spline algorithm is used to generate spline coefficients used in the interpolation. Smooth and accurate field representations are obtained. When vector potential fields are used, the 3D cubic spline interpolation formula analytically generates the magnetic field used to push the particles. This field has del.BETA = 0 to computer roundoff. When magnetic induction is used the interpolation allows del.BETA does not equalmore » 0, which can lead to significant nonphysical results. Presently the code assumes quadrupole symmetry, but this is not an essential feature of the code and could be easily removed for other applications. Many details pertaining to this code are given on microfiche accompanying this report.« less