Novel in vivo imaging techniques for trafficking the behavior of subventricular zone neural stem cells (SVZSC) and SVZSC induced functional repair
Adult progenitor cells hold promise for therapeutic treatment where there has been a disabling loss of function due to death of cells from trauma, disease or aging. However, it will be essential in clinical application to be able to follow the fate of the transplanted cells over time using in vivo tracking methods. We have developed protocol for labeling of progenitor cells to monitor cell trafficking by high resolution magnetic resonance imaging (MRI) and super high resolution positron emission tomography (PET). We have transfected rat subventricular zone stem cells (SVZ, progenitor cell line) and another control cell line (PC12, pheochromocytoma cells) utilizing super paramagnetic iron oxide and poly-L-lysine complex for MR imaging or radiolabeling with 18F-fluor deoxy-D- glucose for PET imaging. The labeled cells were transplanted into the rostral migratory stream (RMS) or striatum of normal or 6-hydroxydopamine lesioned Spraque-Dawley rats. Longitudinal MRI studies (up to 40 days) showed that transplantation site has significant impact to the fate of the cells; when SVZ cells were transplanted into the RMS, cells migrated several centimeter into the olfactory bulb; after transplantation into the striatum, the migration was minimal, only 2 mm. PC 12 cells grew a massive tumor after the striatal implantation and significantly smaller tumor after the RMS implantation. PET studies conducted immediately after transplantation verified the transplantation site. MRI studies were able to show the whole path of migration in one image, since part of the cells die during migration and will get detected because of iron content. Endpoint histological studies verified the cell survival and immunohistochemical studies revealed the differentiation of the transplanted cells into astrocytes and neurons.
- Research Organization:
- Massachusetts General Hospital (US)
- Sponsoring Organization:
- USDOE Office of Science (SC) (US)
- DOE Contract Number:
- FG02-02ER63480
- OSTI ID:
- 819520
- Report Number(s):
- DOE/ER63480; TRN: US0403717
- Resource Relation:
- Other Information: PBD: 28 Nov 2003
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
60 APPLIED LIFE SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS
FUNCTIONALS
GLUCOSE
IN VIVO
INJURIES
IRON OXIDES
MAGNETIC RESONANCE
NEOPLASMS
NERVE CELLS
OLFACTORY BULBS
POSITRONS
REPAIR
RESOLUTION
STEM CELLS
TOMOGRAPHY
PROGENITOR
STEM CELL
TRAFFICKING
MIGRATION
PET
POSITRON EMISSION TOMOGRAPHY
MRI
18F-FDG
SUPER PARAMAGENTIC IRON OXIDE
TRANSFECTION
HSV1
THYMIDINE KINASE