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Title: A fully automated 384 capillary array for DNA sequencer. Final report

Abstract

Phase I SpectruMedix has successfully developed an automatic 96-capillary array DNA prototype based on the multiplexed capillary electrophoresis system originated from Ames Laboratory-USDOE, Iowa State University. With computer control of all steps involved in a 96-capillary array running cycle, the prototype instrument (the SCE9600) is now capable of sequencing 450 base pairs (bp) per capillary, or 48,000 bp per instrument run within 2 hrs. Phase II of this grant involved the advancement of the core 96 capillary technologies, as well as designing a high density 384 capillary prototype. True commercialization of the 96 capillary instrument involved finalization of the gel matrix, streamlining the instrument hardware, creating a more reliable capillary cartridge, and further advancement of the data processing software. Together these silos of technology create a truly commercializable product (the SCE9610) capable of meeting the operation needs of the sequencing centers.

Authors:
;
Publication Date:
Research Org.:
SpectruMedix (US)
Sponsoring Org.:
USDOE Office of Energy Research (ER) (US)
OSTI Identifier:
819022
Report Number(s):
Final Report
TRN: US200503%%819
DOE Contract Number:  
FG02-97ER82461
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: 20 Mar 2003
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; COMMERCIALIZATION; COMPUTERS; DATA PROCESSING; DNA; DNA SEQUENCERS; ELECTROPHORESIS

Citation Formats

Li, Qingbo, and Kane, T. A fully automated 384 capillary array for DNA sequencer. Final report. United States: N. p., 2003. Web. doi:10.2172/819022.
Li, Qingbo, & Kane, T. A fully automated 384 capillary array for DNA sequencer. Final report. United States. https://doi.org/10.2172/819022
Li, Qingbo, and Kane, T. 2003. "A fully automated 384 capillary array for DNA sequencer. Final report". United States. https://doi.org/10.2172/819022. https://www.osti.gov/servlets/purl/819022.
@article{osti_819022,
title = {A fully automated 384 capillary array for DNA sequencer. Final report},
author = {Li, Qingbo and Kane, T},
abstractNote = {Phase I SpectruMedix has successfully developed an automatic 96-capillary array DNA prototype based on the multiplexed capillary electrophoresis system originated from Ames Laboratory-USDOE, Iowa State University. With computer control of all steps involved in a 96-capillary array running cycle, the prototype instrument (the SCE9600) is now capable of sequencing 450 base pairs (bp) per capillary, or 48,000 bp per instrument run within 2 hrs. Phase II of this grant involved the advancement of the core 96 capillary technologies, as well as designing a high density 384 capillary prototype. True commercialization of the 96 capillary instrument involved finalization of the gel matrix, streamlining the instrument hardware, creating a more reliable capillary cartridge, and further advancement of the data processing software. Together these silos of technology create a truly commercializable product (the SCE9610) capable of meeting the operation needs of the sequencing centers.},
doi = {10.2172/819022},
url = {https://www.osti.gov/biblio/819022}, journal = {},
number = ,
volume = ,
place = {United States},
year = {2003},
month = {3}
}