Trapping, detection, and mass determination of coliphage T4 DNA ions of 10{sup 8} Da by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry
- Pacific Northwest Lab., Richland, WA (United States)
The trapping, detection, and mass measurement of individual T4 DNA ions (nominal molecular weight, M{sub r} = 1.1 x 10{sup 8} Da) have been performed with a 7 T electrospray ionization Fourier transform ion cyclotron resonance (ESI-FTICR) mass spectrometer. The ionic mass was obtained by direct measurement of the number of charges carried by individual T4 DNA ions. The ions detected are a factor of approximately 20 larger than any molecule previously studied by mass spectrometry. These gas-phase macroions demonstrate a high degree of stability, which allows the acquisition of long transients (>476 s) from individual ions and the study of relatively slow reactions or dissociation processes. These results show the potential utility of ESI-FTICR mass spectrometry for the analysis of very large biomolecules and microparticles and indicate that it is possible to transfer ions of 110 MDa size to the gas phase substantially intact. The potential applications of the FTICR analysis of such highly charged ions are briefly discussed. 33 refs., 3 figs., 1 tab.
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC06-76RL01830
- OSTI ID:
- 81295
- Journal Information:
- Analytical Chemistry (Washington), Vol. 67, Issue 7; Other Information: PBD: 1 Apr 1995
- Country of Publication:
- United States
- Language:
- English
Similar Records
Direct charge number and molecular weight determination of large individual ions by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry
Automated Gain Control and Internal Calibration With External Ion Accumulation Capillary liquid chromatography-electrospray ionization-fourier transform ion cyclotron resonance.