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Title: LETHALITY OF PSEUDOMONAS FLUORESCENS STRAIN CLO145A TO THE 2 ZEBRA MUSSEL SPECIES PRESENT IN NORTH AMERICA

Abstract

These experiments indicated that bacterial strain CL0145A of Pseudomonas fluorescens is equally lethal to the 2 zebra mussel species present in North America, Dreissena polymorpha and Dreissena bugensis. Thus, this bacterial strain should be equally effective at killing zebra mussels in power plant pipes, irrespective of which species is present.

Authors:
Publication Date:
Research Org.:
New York State Education Department (US)
Sponsoring Org.:
(US)
OSTI Identifier:
811381
DOE Contract Number:
FC26-00NT40751
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: 28 Oct 2001
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 20 FOSSIL-FUELED POWER PLANTS; 21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS; MUSSELS; THERMAL POWER PLANTS; PSEUDOMONAS; STRAINS; TOXICITY

Citation Formats

Daniel P. Molloy. LETHALITY OF PSEUDOMONAS FLUORESCENS STRAIN CLO145A TO THE 2 ZEBRA MUSSEL SPECIES PRESENT IN NORTH AMERICA. United States: N. p., 2001. Web. doi:10.2172/811381.
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Daniel P. Molloy. LETHALITY OF PSEUDOMONAS FLUORESCENS STRAIN CLO145A TO THE 2 ZEBRA MUSSEL SPECIES PRESENT IN NORTH AMERICA. United States. doi:10.2172/811381.
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Daniel P. Molloy. Sun . "LETHALITY OF PSEUDOMONAS FLUORESCENS STRAIN CLO145A TO THE 2 ZEBRA MUSSEL SPECIES PRESENT IN NORTH AMERICA". United States. doi:10.2172/811381. https://www.osti.gov/servlets/purl/811381.
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@article{osti_811381,
title = {LETHALITY OF PSEUDOMONAS FLUORESCENS STRAIN CLO145A TO THE 2 ZEBRA MUSSEL SPECIES PRESENT IN NORTH AMERICA},
author = {Daniel P. Molloy},
abstractNote = {These experiments indicated that bacterial strain CL0145A of Pseudomonas fluorescens is equally lethal to the 2 zebra mussel species present in North America, Dreissena polymorpha and Dreissena bugensis. Thus, this bacterial strain should be equally effective at killing zebra mussels in power plant pipes, irrespective of which species is present.},
doi = {10.2172/811381},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Oct 28 00:00:00 EDT 2001},
month = {Sun Oct 28 00:00:00 EDT 2001}
}
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Technical Report:
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DOI:  10.2172/811381
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  • The specific purpose of this research project was to identify factors that affect zebra mussel kill by the bacterium Pseudomonas fluorescens. Test results obtained during this three-year project identified the following key variables as affecting mussel kill: treatment concentration, treatment duration, mussel siphoning activity, dissolved oxygen concentration, water temperature, and naturally suspended particle load. Using this latter information, the project culminated in a series of pipe tests which achieved high mussel kill inside power plants under once-through conditions using service water in artificial pipes.

  • Under this USDOE-NETL contract, the bacterium Pseudomonas fluorescens is being developed as a biocontrol agent for zebra mussels. The specific purpose of the contract is to identify biotic and abiotic factors that affect mussel kill. Ingestion of these bacteria by zebra mussels is required to achieve kill, and tests evaluating factors that relate to mussel feeding are contained in this report. Specifically the impact of the following two factors were investigated: (1) Mussel siphoning behavior--In nature, zebra mussels typically have their two shells spread apart and their inhalant siphon tube extended from between their shells for taking food particles intomore » their mantle cavities (Fig. 1). Our tests indicated that there is a direct correlation between mussel siphoning activity and mussel mortality achieved by a bacterial treatment. Therefore, to encourage mussel feeding on bacteria, future pipe treatments within power plants should be carried out using procedures which minimize disturbance to mussel siphoning. 2. Naturally suspended particle loads--Since bacterial cells are lethal only if ingested by mussels, waters containing very high levels of naturally suspended particles might reduce the mortality that can be achieved by a bacterial treatment. If true, this inhibition might occur as a result of particle exclusion, i.e., there could be reduced ingestion of bacterial cells since they represent a reduced percentage of all particles ingested. Our tests indicated that a range of particle concentrations that might naturally exist in a turbid river did not inhibit mussel kill by the bacterial cells, but that an artificially high load of natural particles was capable of causing a reduction in kill. To be conservative, therefore, future pipe treatments should be timed to occur when intake waters have relatively low quantities of naturally suspended particulate matter.« less

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  • The blue mussel, Mytilus edulis L. is a widely distributed and locally abundant bivalve mollusc in the North and Mid-Atlantic Regions. It is a valuable commercial species; regional landings in 1986 were worth nearly $4 million. It is a semi-sessile species, anchored by byssus threads to firm surfaces in littoral and sub-littoral environments at salinities ranging from 5 to 35 ppt. It is a suspension feeder, ingesting phytoplankton and detrital particles in the size range of 3--30 /mu/m. The geographical range of the species is limited by lethal water temperatures above 27/degree/C in the south and by temperatures too lowmore » for growth and reproduction in the north. Animals from the northern end of the range are stressed by temperatures above 20/degree/C, whereas those near the southern distributional limit are not severely stressed by temperatures as high as 25/degree/C. The blue mussel is diecious and oviparous. The planktotrophic larvae take about 3 weeks to develop and metamorphose. The environmental tolerances of larvae are more restricted than those of adults. The juveniles grow to approximately 1.5 mm while attached to filamentous algae before being carried by water currents to reattach to a firm substrate, often close to adult mussels. Larval and adult blue mussels are important prey items for many animals, including crabs, fishes, and birds. 95 refs., 5 figs., 2 tabs.« less

  • Over the course of five years we have established a long-term array of warming chambers at Duke and Harvard Forest that simulate future conditions with regard to temperature. In these chambers, we have studied, ants, other animal taxa, fungi, bacteria and plants and their responses to the treatments. We have coupled these studies with lab experiments, large-scale observations, and models to contextualize our results. Finally, we have developed integrative models of the future distribution of species and their consequences as a result of warming in eastern North America and more generally.