Final LDRD Report for the Project Entitled: Biosensors Based on the Electrical Impedance of Tethered Lipid Bilayers on Planar Electrodes

doi:10.2172/809611

Abstract

Impedance based, planar chemical microsensors are the easiest sensors to integrate with electronics. The goal of this work is a several order of magnitude increase in the sensitivity of this sensor type. The basic idea is to mimic biological chemical sensors that rely on changes in ion transport across very thin organic membranes (supported Bilayer Membranes: sBLMs) for the sensing. To improve the durability of bilayers we show how they can be supported on planar metal electrodes. The large increase in sensitivity over polyelectrolytes will come from molecular recognition elements like antibodies that bind the analyte molecule. The molecular recognition sites can be tied to the lipid bilayer capacitor membrane and a number of mechanisms can be used to modulate the impedance of the lipid bilayers. These include coupled ion channels, pore modification and double layer capacitance modification by the analyte molecule. The planar geometry of our electrodes allows us to create arrays of sensors on the same chip, which we are calling the ''Lipid Chip''.

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Song, X.Z. ; Shelnutt, J.A. ; Hobbs, J.D. ; ... February 1997

The objective of this project is the development of a new class of supramolecular assemblies for applications in biosensors and biodevices. The supramolecular assemblies are based on membranes and Langmuir-Blodgett (LB) films composed of naturally-occurring or synthetic lipids, which contain electrically and/or photochemically active components. The LB films are deposited onto electrically-active materials (metal, semiconductors). The active components film components (lipo-porphyrins) at the surface function as molecular recognition sites for sensing proteins and other biomolecules, and the porphyrins and other components (e.g., fullerenes) incorporated into the films serve as photocatalysts and vectorial electron-transport agents. Computer-aided molecular design (CAMD) methods aremore »« less
DOI: 10.2172/461263

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Title: Final LDRD Report for the Project Entitled: Biosensors Based on the Electrical Impedance of Tethered Lipid Bilayers on Planar Electrodes

Abstract

Citation Formats

A progress report on the LDRD project entitled {open_quotes}Microelectronic silicon-based chemical sensors: Ultradetection of high value molecules{close_quotes}

The final LDRD report for the project entitled: {open_quotes}Enhanced analysis of complex gas mixtures by pattern recognition of microsensor array signals{close_quotes}

A Final Report on the Project Entitled, "A Metallic Interconnect for Intermediate Temperature Planar, Solid Oxide Fuel Cells (SOFC)

A Final Report On the Project Entitled LSGM Based Composite Cathodes for Anode Supported, Intermediate Temp. (600-800 degrees C) Solid Oxide Fuel Cells

Designed supramolecular assemblies for biosensors and photoactive devices. LDRD final report