Transposon insertions causing constitutive sex-lethal activity in Drosophila melanogaster affect Sxl sex-specific transcript splicing

Title: Transposon insertions causing constitutive sex-lethal activity in Drosophila melanogaster affect Sxl sex-specific transcript splicing

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Abstract

Sex-lethal (Sxl) gene products induce female development in Drosophila melanogaster and suppress the transcriptional hyperactivation of X-linked genes responsible for male X-chromosome dosage compensation. Control of Sxl functioning by the dose of X-chromosomes normally ensures that the female-specific functions of this developmental switch gene are only expressed in diplo-X individuals. Although the immediate effect of X-chromosome dose is on Sxl transcription, during most of the life cycle {open_quotes}on{close_quotes} vs. {open_quotes}off{close_quotes} reflects alternative Sxl RNA splicing, with the female (productive) splicing mode maintained by a positive feedback activity of SXL protein on Sxl pre-mRNA splicing. {open_quotes}Male-lethal{close_quotes} (Sxl{sup M}) gain-of-function alleles subvert Sxl control by X-chromosome dose, allowing female Sxl functions to be expressed independent of the positive regulators upstream of Sxl. As a consequence, Sxl{sup M} haplo-X animals (chromosomal males) die because of improper dosage compensation, and Sxl{sup m} chromosomal females survive the otherwise lethal effects of mutations in upstream positive regulators. Transcript analysis of double-mutant male-viable Sxl{sup M} derivatives in which the Sxl{sup M} insertion is cis to loss-of-function mutations, combined with other results reported here, indicates that the constitutive character of these Sxl{sup M} alleles is a consequence of an alteration of the structure of the pre-mRNA that allowmore »« less

Authors:

Berstein, M; Cline, T W ^[1]; Lersch, R A; Subrahmanyan, L ^[2]

Princeton Univ., Princeton, NJ (United States)
Univ. of California, Berkeley, CA (United States)

Publication Date:: 1995-02-01

OSTI Identifier:: 79428

Resource Type:: Journal Article

Journal Name:: Genetics

Additional Journal Information:: Journal Volume: 139; Journal Issue: 2; Other Information: PBD: Feb 1995

Country of Publication:: United States

Language:: English

Subject:: 55 BIOLOGY AND MEDICINE, BASIC STUDIES; GENES; TRANSCRIPTION; DROSOPHILA; SEX; X CHROMOSOME; LETHAL GENES; GENE MUTATIONS; RNA; SPLICING; TRANSPOSONS; CELL CYCLE; PROTEINS; MESSENGER-RNA; POLYMERASE CHAIN REACTION; AMINO ACID SEQUENCE

Citation Formats


                    Berstein, M, Cline, T W, Yale Univ., New Haven, CT, Lersch, R A, and Subrahmanyan, L. Transposon insertions causing constitutive sex-lethal activity in Drosophila melanogaster affect Sxl sex-specific transcript splicing.  United States: N. p., 1995. 
        Web.

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                    Berstein, M, Cline, T W, Yale Univ., New Haven, CT, Lersch, R A, & Subrahmanyan, L. Transposon insertions causing constitutive sex-lethal activity in Drosophila melanogaster affect Sxl sex-specific transcript splicing.  United States.

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                    Berstein, M, Cline, T W, Yale Univ., New Haven, CT, Lersch, R A, and Subrahmanyan, L. Wed .  
        "Transposon insertions causing constitutive sex-lethal activity in Drosophila melanogaster affect Sxl sex-specific transcript splicing".  United States.

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@article{osti_79428,

  title        = {Transposon insertions causing constitutive sex-lethal activity in Drosophila melanogaster affect Sxl sex-specific transcript splicing},

  author       = {Berstein, M and Cline, T W and Yale Univ., New Haven, CT and Lersch, R A and Subrahmanyan, L},

  abstractNote = {Sex-lethal (Sxl) gene products induce female development in Drosophila melanogaster and suppress the transcriptional hyperactivation of X-linked genes responsible for male X-chromosome dosage compensation. Control of Sxl functioning by the dose of X-chromosomes normally ensures that the female-specific functions of this developmental switch gene are only expressed in diplo-X individuals. Although the immediate effect of X-chromosome dose is on Sxl transcription, during most of the life cycle {open_quotes}on{close_quotes} vs. {open_quotes}off{close_quotes} reflects alternative Sxl RNA splicing, with the female (productive) splicing mode maintained by a positive feedback activity of SXL protein on Sxl pre-mRNA splicing. {open_quotes}Male-lethal{close_quotes} (Sxl{sup M}) gain-of-function alleles subvert Sxl control by X-chromosome dose, allowing female Sxl functions to be expressed independent of the positive regulators upstream of Sxl. As a consequence, Sxl{sup M} haplo-X animals (chromosomal males) die because of improper dosage compensation, and Sxl{sup m} chromosomal females survive the otherwise lethal effects of mutations in upstream positive regulators. Transcript analysis of double-mutant male-viable Sxl{sup M} derivatives in which the Sxl{sup M} insertion is cis to loss-of-function mutations, combined with other results reported here, indicates that the constitutive character of these Sxl{sup M} alleles is a consequence of an alteration of the structure of the pre-mRNA that allow some level of female splicing to occur even in the absence of functional SXL protein. Surprisingly, however, most of the constitutive character of Sxl{sup M} alleles appears to depend on the mutant alleles` responsiveness, perhaps greater than wild-type, to the autoregulatory splicing activity of the wild-type SXL proteins they produce. 47 refs., 10 figs., 4 tabs.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/79428},
  journal      = {Genetics},
number       = 2,

  volume       = 139,

  place        = {United States},

  year         = {1995},

  month        = {2}

}

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