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Title: Photosynthetic performance of a helical tubular photobioreactor incorporating the cyanobacterium Spirulina platensis

Abstract

The photosynthetic performance of a helical tubular photobioreactor (``Biocoil``), incorporating the filamentous cyanobacterium Spirulina platensis, was investigated. The photobioreactor was constructed in a cylindrical shape with a 0.25-m{sup 2} basal area and a photostage comprising 60 m of transparent PVC tubing of 1.6-cm inner diameter. The inner surface of the cylinder was illuminated with cool white fluorescent lamps; the energy input of photosynthetically active radiation into the photobioreactor was 2,920 kJ per day. An air-lift system incorporating 4% CO{sub 2} was used to circulate the growth medium in the tubing. The maximum productivity achieved in batch culture was 7.18 g dry biomass per day which corresponded to a photosynthetic (PAR) efficiency of 5.45%. The CO{sub 2} was efficiently removed from the gaseous stream; monitoring the CO{sub 2} in the outlet and inlet gas streams showed a 70% removal of CO{sub 2} from the inlet gas over an 8-h period with almost maximum growth rate.

Authors:
;  [1];  [2]
  1. Univ. of London (United Kingdom)
  2. Univ. Laval, Quebec City, Quebec (Canada). Dept. of Food Science and Technology
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
78133
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biotechnology and Bioengineering; Journal Volume: 47; Journal Issue: 2; Other Information: PBD: 20 Jul 1995
Country of Publication:
United States
Language:
English
Subject:
56 BIOLOGY AND MEDICINE, APPLIED STUDIES; CYANOBACTERIA; CARBON DIOXIDE FIXATION; BIOREACTORS; PERFORMANCE; GREENHOUSE EFFECT; PHOTOSYNTHESIS

Citation Formats

Watanabe, Yoshitomo, Hall, D.O., and Nouee, J. De La. Photosynthetic performance of a helical tubular photobioreactor incorporating the cyanobacterium Spirulina platensis. United States: N. p., 1995. Web. doi:10.1002/bit.260470218.
Watanabe, Yoshitomo, Hall, D.O., & Nouee, J. De La. Photosynthetic performance of a helical tubular photobioreactor incorporating the cyanobacterium Spirulina platensis. United States. doi:10.1002/bit.260470218.
Watanabe, Yoshitomo, Hall, D.O., and Nouee, J. De La. 1995. "Photosynthetic performance of a helical tubular photobioreactor incorporating the cyanobacterium Spirulina platensis". United States. doi:10.1002/bit.260470218.
@article{osti_78133,
title = {Photosynthetic performance of a helical tubular photobioreactor incorporating the cyanobacterium Spirulina platensis},
author = {Watanabe, Yoshitomo and Hall, D.O. and Nouee, J. De La},
abstractNote = {The photosynthetic performance of a helical tubular photobioreactor (``Biocoil``), incorporating the filamentous cyanobacterium Spirulina platensis, was investigated. The photobioreactor was constructed in a cylindrical shape with a 0.25-m{sup 2} basal area and a photostage comprising 60 m of transparent PVC tubing of 1.6-cm inner diameter. The inner surface of the cylinder was illuminated with cool white fluorescent lamps; the energy input of photosynthetically active radiation into the photobioreactor was 2,920 kJ per day. An air-lift system incorporating 4% CO{sub 2} was used to circulate the growth medium in the tubing. The maximum productivity achieved in batch culture was 7.18 g dry biomass per day which corresponded to a photosynthetic (PAR) efficiency of 5.45%. The CO{sub 2} was efficiently removed from the gaseous stream; monitoring the CO{sub 2} in the outlet and inlet gas streams showed a 70% removal of CO{sub 2} from the inlet gas over an 8-h period with almost maximum growth rate.},
doi = {10.1002/bit.260470218},
journal = {Biotechnology and Bioengineering},
number = 2,
volume = 47,
place = {United States},
year = 1995,
month = 7
}
  • The ribulose diphosphate (RDP) carboxylase activity of the cyanobacterium Spirulina platensis is represented by two peaks when a cell homogenate is centrifuged in a sucrose density gradient. In the case of differential centrifugation (40,000 g, 1 h), the activity of the enzyme was distributed between the supernatant liquid (soluble form) and the precipitate (carboxysomal form). From the soluble fraction, in which 80-95% of the total activity of the enzyme is concentrated, electrophoretically homogeneous RDP carboxylase was isolated by precipitation with ammonium sulfate and centrifugation in a sucrose density gradient. The purified enzyme possessed greater electrophoretic mobility in comparison with themore » RDP carboxylase of beans Vicia faba. The molecular weight of the enzyme, determined by gel filtration, was 450,000. The enzyme consists of monotypic subunits with a molecular weight of 53,000. The small subunits were not detected in electrophoresis in polyacrylamide gel in the presence of SDS after fixation and staining of the gels by various methods.« less
  • The characteristics of the protein of fresh-water, mass-cultured Spirulina platensis have been studied. The solubility of this algal protein in water and various aqueous solvents has been estimated. The total protein content of the blue-green algae was approximately 50-55% of which nearly 9.9% was nonprotein nitrogen. About 80% of the total protein nitrogen can be extracted by three successive extractions with water. Ths isoelectric point of this algal protein is found to be 3.0. The total proteins were characterized physicochemically by standard techniques. In the ultracentrifuge total proteins resolve into two major components with S20w values of 2.6 and 4.7more » S. The polyacrylamide gel electrophoretic pattern of the total protein showed seven bands including three prominent ones. The in vitro digestibility of the total protein of fresh algae was found to be 85% when assayed with a pepsin-pancreatin system.« less
  • Cobalt, a micronutrient for biological organisms, is a metal of wide use. Main sources of Co to the environment are combustion of fossil fuels, smelters, cobalt processing facilities, sewage and industrial wastes. Atomic power plants and nuclear weapon detonations form an important source of radioisotopes of this metal to the environment. Cobalt has been included in the 14 toxic trace elements of critical importance from the point of view of environmental pollution and health hazards. Cobalt deficiency leads to diseases like stunted growth. At toxic level, Co inhibits heme biosynthesis and enzyme activities. The present study reports the effect ofmore » cobalt on biomass productivity of blue-green alga Spirulina platensis.« less
  • No abstract prepared.