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Title: Ligand interaction with the purified serotonin transporter in solution and at the air/water interface

Abstract

The purified serotonin transporter (SERT) was spread at the air/water interface and the effects both of its surface density and of the temperature on its interfacial behavior were studied. The recorded isotherms evidenced the existence of a stable monolayer undergoing a lengthy rearrangement. SERT/ligand interactions appeared to be dependent on the nature of the studied molecules. Whereas an unrelated drug (chlorcyclizine) did not bind to the spread SERT, it interacted with its specific ligands. Compared to heterocyclic drugs, for which binding appeared to be concentration-dependent, a 'two-site' mechanism was evidenced for pinoline and imipramine.

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab., CA (US)
Sponsoring Org.:
National Institutes of Health (US)
OSTI Identifier:
776624
Report Number(s):
LBNL-46403
Journal ID: ISSN 0014-5793; FEBLAL; R&D Project: 865036; TRN: AH200118%%462
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Journal Article
Journal Name:
FEBS Letters
Additional Journal Information:
Journal Volume: 471; Journal Issue: 1; Other Information: Journal Publication Date: Apr. 7, 2000; PBD: 1 Jun 2000; Journal ID: ISSN 0014-5793
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; IMIPRAMINE; ADSORPTION ISOTHERMS; SEROTONIN; INTERFACES; LIGANDS; INTERACTIONS; SURFACE PROPERTIES; DRUGS; BIOLOGICAL EFFECTS; TRANSPORTER; SURFACE PRESSURE; PROTEIN MONOLAYER; SEROTONIN TRANSPORTER-DRUG INTERACTION

Citation Formats

Faivre, V., Manivet, P., Callaway, J.C., Morimoto, H., Airaksinen, M.M., Baszkin, A., Launay, J.M., and Rosilio, V. Ligand interaction with the purified serotonin transporter in solution and at the air/water interface. United States: N. p., 2000. Web. doi:10.1016/S0014-5793(00)01362-4.
Faivre, V., Manivet, P., Callaway, J.C., Morimoto, H., Airaksinen, M.M., Baszkin, A., Launay, J.M., & Rosilio, V. Ligand interaction with the purified serotonin transporter in solution and at the air/water interface. United States. doi:10.1016/S0014-5793(00)01362-4.
Faivre, V., Manivet, P., Callaway, J.C., Morimoto, H., Airaksinen, M.M., Baszkin, A., Launay, J.M., and Rosilio, V. Thu . "Ligand interaction with the purified serotonin transporter in solution and at the air/water interface". United States. doi:10.1016/S0014-5793(00)01362-4.
@article{osti_776624,
title = {Ligand interaction with the purified serotonin transporter in solution and at the air/water interface},
author = {Faivre, V. and Manivet, P. and Callaway, J.C. and Morimoto, H. and Airaksinen, M.M. and Baszkin, A. and Launay, J.M. and Rosilio, V.},
abstractNote = {The purified serotonin transporter (SERT) was spread at the air/water interface and the effects both of its surface density and of the temperature on its interfacial behavior were studied. The recorded isotherms evidenced the existence of a stable monolayer undergoing a lengthy rearrangement. SERT/ligand interactions appeared to be dependent on the nature of the studied molecules. Whereas an unrelated drug (chlorcyclizine) did not bind to the spread SERT, it interacted with its specific ligands. Compared to heterocyclic drugs, for which binding appeared to be concentration-dependent, a 'two-site' mechanism was evidenced for pinoline and imipramine.},
doi = {10.1016/S0014-5793(00)01362-4},
journal = {FEBS Letters},
issn = {0014-5793},
number = 1,
volume = 471,
place = {United States},
year = {2000},
month = {6}
}