Low-dose responses to 2,3,7,8-tetrachlorodibenzo-p-dioxin in single living human cells measured by synhrotron infrared spectromicroscopy
We report synchrotron radiation-based (SR) Fourier-transform infrared (FTIR) spectromicroscopy measurements of HepG2 cells exposed to an environmental contaminant that is a known ligand for the aryl hydrocarbon receptor (AhR). Measurements were made on cells treated with an AhR-binding model compound 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Compared to untreated control cells, treated cells display unique spectral changes with TCDD concentrations of 0.01 to 10.0 nanomolar (nM). Key spectral changes involve P=O, C-O and C-H stretching bands. The first changes are related to the environment of the phosphate backbone of nucleic acids. The C-H stretching bands data show a relative increase in the number of methyl to methylene groups. We find an excellent correlation between spectral changes and an increase in CYP1A1 expression measured by the reverse transcriptase polymerase chain reaction (RT-PCR) technique demonstrating that the SR-FTIR method is observing cellular biochemical changes related to this gene expression pathway. Finally, we discuss the potential use of SR-FTIR spectromicroscopy as a diagnostic tool for monitoring cellular exposure and early molecular responses to environmental pollutants.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE Director, Office of Science (US)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 767528
- Report Number(s):
- LBNL-43783; R&D Project: 523601; TRN: US0005698
- Journal Information:
- Environmental Science & Technology, Vol. 34, Issue 12; Other Information: PBD: 12 Dec 1999
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
GENES
HYDROCARBONS
MONITORING
NUCLEIC ACIDS
PHOSPHATES
POLLUTANTS
POLYMERASE CHAIN REACTION
SYNCHROTRONS
2
3
7
8-TETRACHLORODIBENZO-P-DIOXIN TCDD AHR ARYL HYDROCARBON RECEPTOR CELLULAR RESPONSE INFRARED SPECTROSCOPY SYNCHROTRON FTIR SPECTROMICROSCOPY
EPTOR CELLULAR RESPONSE INFRARED SPECTROSCOPY SYNCHROTRON FTIR SPECTROMICROSCOPY