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Title: Final Report: Regulation and Function of Two Cell Wall protein Genes in Me Dicago Roots and Root Nodules, August 1, 1995 - January 31, 1999

Abstract

During the period of DOE funding we synthesized several PRP peptides, generated rabbit antisera against two PRP repeats found in early nodulin PRPs, and developed confocal microscopy methods for root immunohistochemistry. Using the antibodies, we completed extensive descriptive studies of PRP deposition in medic and alfalfa roots showing that PRPs deposition is developmentally regulated in roots and spatially restricted within the walls of specific root tissues. Domain-specific antibodies were isolated from polyclonal sera using peptide affinity chromatography and were then used to demonstrate that nodule-specific epitopes are shared by several nodule-specific proteins. The following provides a more detailed summary of this work.

Authors:
Publication Date:
Research Org.:
University of California, Santa Barbara, CA (US)
Sponsoring Org.:
USDOE Office of Energy Research (ER) (US)
OSTI Identifier:
765642
DOE Contract Number:  
FG03-95ER20192
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: 8 May 2000
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; AFFINITY; ALFALFA; ANTIBODIES; CELL WALL; CHROMATOGRAPHY; DEPOSITION; GENES; MICROSCOPY; PEPTIDES; PROTEINS; RABBITS; REGULATIONS

Citation Formats

Cooper, James B. Final Report: Regulation and Function of Two Cell Wall protein Genes in Me Dicago Roots and Root Nodules, August 1, 1995 - January 31, 1999. United States: N. p., 2000. Web. doi:10.2172/765642.
Cooper, James B. Final Report: Regulation and Function of Two Cell Wall protein Genes in Me Dicago Roots and Root Nodules, August 1, 1995 - January 31, 1999. United States. doi:10.2172/765642.
Cooper, James B. Mon . "Final Report: Regulation and Function of Two Cell Wall protein Genes in Me Dicago Roots and Root Nodules, August 1, 1995 - January 31, 1999". United States. doi:10.2172/765642. https://www.osti.gov/servlets/purl/765642.
@article{osti_765642,
title = {Final Report: Regulation and Function of Two Cell Wall protein Genes in Me Dicago Roots and Root Nodules, August 1, 1995 - January 31, 1999},
author = {Cooper, James B.},
abstractNote = {During the period of DOE funding we synthesized several PRP peptides, generated rabbit antisera against two PRP repeats found in early nodulin PRPs, and developed confocal microscopy methods for root immunohistochemistry. Using the antibodies, we completed extensive descriptive studies of PRP deposition in medic and alfalfa roots showing that PRPs deposition is developmentally regulated in roots and spatially restricted within the walls of specific root tissues. Domain-specific antibodies were isolated from polyclonal sera using peptide affinity chromatography and were then used to demonstrate that nodule-specific epitopes are shared by several nodule-specific proteins. The following provides a more detailed summary of this work.},
doi = {10.2172/765642},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {5}
}