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Title: Molecular analysis of a thylakoid K+channel

Abstract

The work undertaken sought to use a novel probe to identify and clone plant ion (K) channels. It was also proposed that in vitro biochemical studies of cation transport across purified preparations of thylakoid membrane be employed to characterize a putative K channel in this membrane system. Over the last several years, an enormous data base of partially-sequenced mRNAs and numerous genomes (including those of plants) has evolved and provides a powerful alternative to this brute-force approach to identify and clone cDNAs encoding physiologically important membrane proteins such as channels. The utility of searching genetic databases for relevant sequences, in addition to the difficulty of working with membrane proteins, led to changes in research focus during the granting period. During the course of the funding period, work was finished up which documented the presence of a K channel in the thylakoid membrane and demonstrated that K fluxes through this channel were required for optimal photosynthetic activity, likely due to the requirement for charge balancing of proton flux.

Publication Date:
Research Org.:
Rutgers Univ., New Brunswick, New Jersey (US)
Sponsoring Org.:
USDOE Office of Energy Research (ER) (US)
OSTI Identifier:
764703
DOE Contract Number:  
FG02-95ER20202
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: 10 Sep 1999
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS; CATIONS; GENETICS; IN VITRO; MEMBRANES; PROBES; PROTONS; TRANSPORT

Citation Formats

NONE. Molecular analysis of a thylakoid K+channel. United States: N. p., 1999. Web. doi:10.2172/764703.
NONE. Molecular analysis of a thylakoid K+channel. United States. doi:10.2172/764703.
NONE. Fri . "Molecular analysis of a thylakoid K+channel". United States. doi:10.2172/764703. https://www.osti.gov/servlets/purl/764703.
@article{osti_764703,
title = {Molecular analysis of a thylakoid K+channel},
author = {NONE},
abstractNote = {The work undertaken sought to use a novel probe to identify and clone plant ion (K) channels. It was also proposed that in vitro biochemical studies of cation transport across purified preparations of thylakoid membrane be employed to characterize a putative K channel in this membrane system. Over the last several years, an enormous data base of partially-sequenced mRNAs and numerous genomes (including those of plants) has evolved and provides a powerful alternative to this brute-force approach to identify and clone cDNAs encoding physiologically important membrane proteins such as channels. The utility of searching genetic databases for relevant sequences, in addition to the difficulty of working with membrane proteins, led to changes in research focus during the granting period. During the course of the funding period, work was finished up which documented the presence of a K channel in the thylakoid membrane and demonstrated that K fluxes through this channel were required for optimal photosynthetic activity, likely due to the requirement for charge balancing of proton flux.},
doi = {10.2172/764703},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {9}
}