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Title: Molecular analysis of a thylakoid K+ channel

Abstract

The work undertaken during the prior granting period sought to use a novel probe to identify and clone plant ion (K) channels. It was also proposed that in vitro biochemical studies of cation transport across purified preparations of thylakoid membrane be employed to characterize a putative K channel in this membrane system. Over the last several years (including those of the previous grant period), an enormous data base of partially-sequenced mRNAs and numerous genomes (including those of plants) has evolved and provides a powerful alternative to this brute-force approach to identify and clone cDNAs ending physiologically important membrane proteins such as channels. The utility of searching genetic databases for relevant sequences, in addition to the difficulty of working with membrane proteins, led to changes in research focus during the prior granting period, and has resulted in the identification of a new class of plant ion channels, which will be the focus of research during the proposed new granting period.

Publication Date:
Research Org.:
University of Connecticut, Storrs, CT (US)
Sponsoring Org.:
US Department of Energy (US)
OSTI Identifier:
764622
DOE Contract Number:  
FG02-97ER20291
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: 1 May 2000
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CATIONS; GENETICS; IN VITRO; MEMBRANES; PROBES; TRANSPORT

Citation Formats

NONE. Molecular analysis of a thylakoid K+ channel. United States: N. p., 2000. Web. doi:10.2172/764622.
NONE. Molecular analysis of a thylakoid K+ channel. United States. doi:10.2172/764622.
NONE. Mon . "Molecular analysis of a thylakoid K+ channel". United States. doi:10.2172/764622. https://www.osti.gov/servlets/purl/764622.
@article{osti_764622,
title = {Molecular analysis of a thylakoid K+ channel},
author = {NONE},
abstractNote = {The work undertaken during the prior granting period sought to use a novel probe to identify and clone plant ion (K) channels. It was also proposed that in vitro biochemical studies of cation transport across purified preparations of thylakoid membrane be employed to characterize a putative K channel in this membrane system. Over the last several years (including those of the previous grant period), an enormous data base of partially-sequenced mRNAs and numerous genomes (including those of plants) has evolved and provides a powerful alternative to this brute-force approach to identify and clone cDNAs ending physiologically important membrane proteins such as channels. The utility of searching genetic databases for relevant sequences, in addition to the difficulty of working with membrane proteins, led to changes in research focus during the prior granting period, and has resulted in the identification of a new class of plant ion channels, which will be the focus of research during the proposed new granting period.},
doi = {10.2172/764622},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {5}
}