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Title: Irradiation of Microbes from Spent Nuclear Fuel Storage Pool Environments

Abstract

Microbes have been isolated and identified from spent nuclear fuel storage pools at the Idaho National Engineering and Environmental Laboratory (INEEL). Included among these are Corynebacterium aquaticum, Pseudomonas putida, Comamonas acidovorans, Gluconobacter cerinus, Micrococcus diversus, Rhodococcus rhodochrous, and two strains of sulfate-reducing bacteria (SRB). We examined the sensitivity of these microbes to a variety of total exposures of radiation generated by a 6-MeV linear accelerator (LINAC). The advantage of using a LINAC is that it provides a relatively quick screen of radiation tolerance. In the first set of experiments, we exposed each of the aforementioned microbes along with four additional microbes, pseudomonas aeruginosa, Micrococcus luteus, Escherchia coli, and Deinococcus radiodurans to exposures of 5 x 10{sup 3} and 6 x 10{sup 4} rad. All microbial specimens withstood the lower exposure with little or no reduction in cell population. Upon exposing the microbes to the larger dose of 6 x 10{sup 4} rad, we observed two distinct groupings: microbes that demonstrate resistance to radiation, and microbes that display intolerance through a dramatic reduction from their initial population. Microbes in the radiation tolerant grouping were exposed to 1.1 x 10{sup 5} rad to examine the extent of their resistance. We observe amore » correlation between radiation resistance and gram stain. The gram-positive species we examined seem to demonstrate a greater radiation resistance.« less

Authors:
; ; ; ; ;  [1];  [2]; ;
  1. INEEL (US)
  2. ISU (US)
Publication Date:
Research Org.:
Idaho National Engineering and Environmental Lab., Idaho Falls, ID (US)
Sponsoring Org.:
USDOE Office of Environmental Management (EM) (US)
OSTI Identifier:
7571
Report Number(s):
INEEL/CON-99-00611
TRN: US0101796
DOE Contract Number:  
AC07-94ID13223
Resource Type:
Conference
Resource Relation:
Conference: Global '99 International Conference on Future Nuclear Systems, Jackson Hole, WY (US), 08/29/1099--09/03/1999; Other Information: PBD: 3 Sep 1999
Country of Publication:
United States
Language:
English
Subject:
11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS; 61 RADIATION PROTECTION AND DOSIMETRY; IRRADIATION; MICROCOCCUS LUTEUS; SPENT FUEL STORAGE; SULFATE-REDUCING BACTERIA; PSEUDOMONAS; RHODOCOCCUS; FUEL STORAGE POOLS; MICROORGANISMS; RADIATION DOSES; SPENT NUCLEAR FUEL STORAGE POOLS; CORYNEBACTERIUM AQUATICUM; PSEUDOMONAS PUTIDA; COMAMONAS ACIDOVORANS; GLUCONOBACTER CERINUS; MICROCOCCUS DIVERSUS; RHODOCOCCUS RHOLOCHROUS; MICROBES; SULFATE-REDUCING BACTERIA (SRB); RADIATION TOLERANCE; CELL POPULATION; GRAM STAIN

Citation Formats

Breckenridge, C.R., Watkins, C.S., Bruhn, D.F., Roberto, F.F., Tsang, M.N., Pinhero, P.J., Brey, R.F., Wright, R.N., and Windes, W.F. Irradiation of Microbes from Spent Nuclear Fuel Storage Pool Environments. United States: N. p., 1999. Web.
Breckenridge, C.R., Watkins, C.S., Bruhn, D.F., Roberto, F.F., Tsang, M.N., Pinhero, P.J., Brey, R.F., Wright, R.N., & Windes, W.F. Irradiation of Microbes from Spent Nuclear Fuel Storage Pool Environments. United States.
Breckenridge, C.R., Watkins, C.S., Bruhn, D.F., Roberto, F.F., Tsang, M.N., Pinhero, P.J., Brey, R.F., Wright, R.N., and Windes, W.F. Fri . "Irradiation of Microbes from Spent Nuclear Fuel Storage Pool Environments". United States. https://www.osti.gov/servlets/purl/7571.
@article{osti_7571,
title = {Irradiation of Microbes from Spent Nuclear Fuel Storage Pool Environments},
author = {Breckenridge, C.R. and Watkins, C.S. and Bruhn, D.F. and Roberto, F.F. and Tsang, M.N. and Pinhero, P.J. and Brey, R.F. and Wright, R.N. and Windes, W.F.},
abstractNote = {Microbes have been isolated and identified from spent nuclear fuel storage pools at the Idaho National Engineering and Environmental Laboratory (INEEL). Included among these are Corynebacterium aquaticum, Pseudomonas putida, Comamonas acidovorans, Gluconobacter cerinus, Micrococcus diversus, Rhodococcus rhodochrous, and two strains of sulfate-reducing bacteria (SRB). We examined the sensitivity of these microbes to a variety of total exposures of radiation generated by a 6-MeV linear accelerator (LINAC). The advantage of using a LINAC is that it provides a relatively quick screen of radiation tolerance. In the first set of experiments, we exposed each of the aforementioned microbes along with four additional microbes, pseudomonas aeruginosa, Micrococcus luteus, Escherchia coli, and Deinococcus radiodurans to exposures of 5 x 10{sup 3} and 6 x 10{sup 4} rad. All microbial specimens withstood the lower exposure with little or no reduction in cell population. Upon exposing the microbes to the larger dose of 6 x 10{sup 4} rad, we observed two distinct groupings: microbes that demonstrate resistance to radiation, and microbes that display intolerance through a dramatic reduction from their initial population. Microbes in the radiation tolerant grouping were exposed to 1.1 x 10{sup 5} rad to examine the extent of their resistance. We observe a correlation between radiation resistance and gram stain. The gram-positive species we examined seem to demonstrate a greater radiation resistance.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {9}
}

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