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Title: Linkage map of the honey bee, Apis mellifera, based on RAPD markers

Abstract

A linkage map was constructed for the honey bee based on the segregation of 365 random amplified polymorphic DNA (RAPD) markers in haploid male progeny of a single female bee. The X locus for sex determination and genes for black body color and malate dehydrogenase were mapped to separate linkage groups. RAPD markers were very efficient for mapping, with an average of about 2.8 loci mapped for each 10-nucleotide primer that was used in polymerase chain reactions. The mean interval size between markers on the map was 9.1 cM. The map covered 3110 cM of linked markers on 26 linkage groups. We estimate the total genome size to be {approximately}3450 cM. The size of the map indicated a very high recombination rate for the honey bee. The relationship of physical to genetic distance was estimated at 52 kb/cM, suggesting that map-based cloning of genes will be feasible for this species. 71 refs., 6 figs., 1 tab.

Authors:
;  [1]
  1. Univ. of California, Davis, CA (United States)
Publication Date:
OSTI Identifier:
75597
Resource Type:
Journal Article
Journal Name:
Genetics
Additional Journal Information:
Journal Volume: 139; Journal Issue: 3; Other Information: PBD: Mar 1995
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; BEES; BEHAVIOR; BIOLOGICAL MARKERS; SIZE; CHROMOSOMES; GENETIC MAPPING; GENE RECOMBINATION; DNA-CLONING; NUCLEOTIDES; POLYMERASE CHAIN REACTION; ELECTROPHORESIS; COMPUTER CODES; GENES

Citation Formats

Hunt, G J, and Page, Jr, R E. Linkage map of the honey bee, Apis mellifera, based on RAPD markers. United States: N. p., 1995. Web.
Hunt, G J, & Page, Jr, R E. Linkage map of the honey bee, Apis mellifera, based on RAPD markers. United States.
Hunt, G J, and Page, Jr, R E. Wed . "Linkage map of the honey bee, Apis mellifera, based on RAPD markers". United States.
@article{osti_75597,
title = {Linkage map of the honey bee, Apis mellifera, based on RAPD markers},
author = {Hunt, G J and Page, Jr, R E},
abstractNote = {A linkage map was constructed for the honey bee based on the segregation of 365 random amplified polymorphic DNA (RAPD) markers in haploid male progeny of a single female bee. The X locus for sex determination and genes for black body color and malate dehydrogenase were mapped to separate linkage groups. RAPD markers were very efficient for mapping, with an average of about 2.8 loci mapped for each 10-nucleotide primer that was used in polymerase chain reactions. The mean interval size between markers on the map was 9.1 cM. The map covered 3110 cM of linked markers on 26 linkage groups. We estimate the total genome size to be {approximately}3450 cM. The size of the map indicated a very high recombination rate for the honey bee. The relationship of physical to genetic distance was estimated at 52 kb/cM, suggesting that map-based cloning of genes will be feasible for this species. 71 refs., 6 figs., 1 tab.},
doi = {},
url = {https://www.osti.gov/biblio/75597}, journal = {Genetics},
number = 3,
volume = 139,
place = {United States},
year = {1995},
month = {3}
}