The G{sub 1} process in PHA-stimulated human lymphocytes and the appearance of radiation-induced chromosome aberrations

Title: The G{sub 1} process in PHA-stimulated human lymphocytes and the appearance of radiation-induced chromosome aberrations

Full Record
Other Related Research

Abstract

Experimental evidence for the existence of local spontaneous DNA synthesis in PHA-stimulated human lymphocytes at the G{sub 1} phase (G{sub 1} process) is presented. This process is associated with the appearance of single-stranded DNA breaks and their subsequent reparation. DNA sequences involved in the G{sub 1} process were characterized in terms of their nucleotide composition and association with the nuclear matrix. It was shown that the G{sub 1} process occurs in chromosome regions in which radiation-induced exchange-type chromosome aberrations arise. The results of the study are regarded as evidence in favor of a molecular version of the primary contact theory of chromosomal mutagenesis. A possible role of G{sub 1} process disturbances in pathogenesis of chromosome instability syndromes in humans is discussed. 18 refs., 3 figs., 2 tabs.

Authors:

Akif`ev, A P; Khudolii, G A; Krasnopevtsev, A V ^[1]; Yakimenko, A V; Khandogina, E K ^[2]

Semenov Institute of Chemical Physics, Moscow (Russian Federation)
Russian Medical State Univ., Moscow (Russian Federation)

Publication Date:: 1995-04-01

OSTI Identifier:: 75554

Resource Type:: Journal Article

Journal Name:: Russian Journal of Genetics

Additional Journal Information:: Journal Volume: 31; Journal Issue: 4; Other Information: PBD: Apr 1995; TN: Translated from Genetika; 31: No. 4, 485-491(1995)

Country of Publication:: United States

Language:: English

Subject:: 55 BIOLOGY AND MEDICINE, BASIC STUDIES; 56 BIOLOGY AND MEDICINE, APPLIED STUDIES; CHROMOSOMES; CHROMOSOMAL ABERRATIONS; MUTAGENESIS; GENES; BIOLOGICAL RADIATION EFFECTS; DNA; SYNTHESIS; LYMPHOCYTES; NUCLEOTIDES

Citation Formats


                    Akif`ev, A P, Khudolii, G A, Krasnopevtsev, A V, Yakimenko, A V, and Khandogina, E K. The G{sub 1} process in PHA-stimulated human lymphocytes and the appearance of radiation-induced chromosome aberrations.  United States: N. p., 1995. 
        Web.

Copy to clipboard


                    Akif`ev, A P, Khudolii, G A, Krasnopevtsev, A V, Yakimenko, A V, & Khandogina, E K. The G{sub 1} process in PHA-stimulated human lymphocytes and the appearance of radiation-induced chromosome aberrations.  United States.

Copy to clipboard


                    Akif`ev, A P, Khudolii, G A, Krasnopevtsev, A V, Yakimenko, A V, and Khandogina, E K. Sat .  
        "The G{sub 1} process in PHA-stimulated human lymphocytes and the appearance of radiation-induced chromosome aberrations".  United States.

Copy to clipboard


                    
@article{osti_75554,

  title        = {The G{sub 1} process in PHA-stimulated human lymphocytes and the appearance of radiation-induced chromosome aberrations},

  author       = {Akif`ev, A P and Khudolii, G A and Krasnopevtsev, A V and Yakimenko, A V and Khandogina, E K},

  abstractNote = {Experimental evidence for the existence of local spontaneous DNA synthesis in PHA-stimulated human lymphocytes at the G{sub 1} phase (G{sub 1} process) is presented. This process is associated with the appearance of single-stranded DNA breaks and their subsequent reparation. DNA sequences involved in the G{sub 1} process were characterized in terms of their nucleotide composition and association with the nuclear matrix. It was shown that the G{sub 1} process occurs in chromosome regions in which radiation-induced exchange-type chromosome aberrations arise. The results of the study are regarded as evidence in favor of a molecular version of the primary contact theory of chromosomal mutagenesis. A possible role of G{sub 1} process disturbances in pathogenesis of chromosome instability syndromes in humans is discussed. 18 refs., 3 figs., 2 tabs.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/75554},
  journal      = {Russian Journal of Genetics},
number       = 4,

  volume       = 31,

  place        = {United States},

  year         = {1995},

  month        = {4}

}

Copy to clipboard

Journal Article:

Other availability

Find in Google Scholar

Search WorldCat to find libraries that may hold this journal

Save / Share:

Export Metadata

Save to My Library

Similar records in OSTI.GOV collections:

DNA repair and chromosome aberrations: the effect of cytosine arabinoside on the frequency of chromosome aberrations induced by radiation and chemicals

Journal Article Preston, R J - Teratog., Carcinog., Mutagen.; (United States)

The frequency of x-ray-induced chromosome aberrations in G/sub 0/ human lymphocytes was greatly increased when cells were incubated with cytosine arabinoside (ara-C) after irradiation. The frequency of dicentrics increased with increasing ara-C incubation times (one, two, and three hours). Lymphocytes from Down syndrome individuals were more sensitive to aberration induction by x-rays in G/sub 0/, and the increase in dicentric frequency with ara-C incubation was much more rapid than with normal cells. When G/sub 2/ normal lymphocytes were x-irradiated and incubated for two or three hours with ara-C until fixation, there was a large increase in depletion frequency compared tomore »« less
Rapid metaphase and interphase detection of radiation-induced chromosome aberrations in human lymphocytes by chromosomal suppression in situ hybridization

Journal Article Cremer, T ; Popp, S ; Emmerich, P ; ... - Cytometry (Baltimore); (USA)

Chromosomal in situ suppression (CISS)-hybridization of biotinylated phage DNA-library inserts from sorted human chromosomes was used to decorate chromosomes 1 and 7 specifically from pter to qter and to detect structural aberrations of these chromosomes in irradiated human peripheral lymphocytes. In addition, probe pUC1.77 was used to mark the 1q12 subregion in normal and aberrant chromosomes 1. Low LET radiation (60Co-gamma-rays; 1.17 and 1.33 MeV) of lymphocyte cultures was performed with various doses (D = 0, 2, 4, 8 Gy) 5 h after stimulation with phytohaemagglutinin. Irradiated cells were cultivated for an additional 67 h before Colcemid arrested metaphase spreadsmore »« less
https://doi.org/10.1002/cyto.990110113
Production of chromosome aberrations in various mammalian cells by triethylenemelamine

Journal Article Luippold, H E ; Gooch, P C ; Brewen, J G - Genetics; (United States)

The cytogenetic effects of triethylenemelamine (TEM) were studied using five different mammalian tissues. Treatments of 0.1 and 0.2 mg/kg TEM on differentiating mouse spermatogonia and bone marrow cells showed no significant differences in the frequency of chromosomal aberrations produced in these two tissues. At higher doses, however, the sensitivities of the two tissues appear to be different. The frequency of aberrations varies with time after treatment, with the greatest amount occurring at the later fixation times. Results of an experiment on primary spermatocytes indicated a correlation between the frequency of chromosome aberrations and DNA replication. Human peripheral leukocytes were utilizedmore »« less
X-ray-induced chromosome aberrations in Down lymphocytes: an explanation of their increased sensitivity

Journal Article Preston, R J - Environ. Mutagen.; (United States)

Unstimulated lymphocytes from individuals with Down Syndrome (trisomy 21) are more sensitive to the induction of dicentric and ring aberrations by X rays than normal lymphocytes. Several explanations involving the more rapid rejoining of X-ray--induced lesions in Down cells have been offered. It is shown here that the repair of the DNA damage converted into chromosome aberrations is more rapid in Down cells than normal cells. This more rapid repair results in a higher probability of producing chromosomes aberrations, and hence higher aberration frequencies in Down than normal cells.
https://doi.org/10.1002/em.2860030109
X-ray-induced chromosome aberrations in Down lymphocytes: an explanation of their increased sensitivity

Journal Article Preston, R J - Environ. Mutagen.; (United States)

Unstimulated lymphocytes from individuals with Down Syndrome (trisomy 21) are more sensitive to the induction of dicentric and ring aberrations by X rays than normal lymphocytes. Several explanations involving the more rapid rejoining of X-ray-induced lesions in Down cells have been offered. It is shown here that the repair of the DNA damage converted into chromosome aberrations is more rapid in Down cells than normal cells. This more rapid repair results in a higher probability of producing chromosome aberrations, and hence higher aberration frequencies in Down than normal cells.
https://doi.org/10.1002/em.2860030109

Similar Records