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Title: The G{sub 1} process in PHA-stimulated human lymphocytes and the appearance of radiation-induced chromosome aberrations

Abstract

Experimental evidence for the existence of local spontaneous DNA synthesis in PHA-stimulated human lymphocytes at the G{sub 1} phase (G{sub 1} process) is presented. This process is associated with the appearance of single-stranded DNA breaks and their subsequent reparation. DNA sequences involved in the G{sub 1} process were characterized in terms of their nucleotide composition and association with the nuclear matrix. It was shown that the G{sub 1} process occurs in chromosome regions in which radiation-induced exchange-type chromosome aberrations arise. The results of the study are regarded as evidence in favor of a molecular version of the primary contact theory of chromosomal mutagenesis. A possible role of G{sub 1} process disturbances in pathogenesis of chromosome instability syndromes in humans is discussed. 18 refs., 3 figs., 2 tabs.

Authors:
; ;  [1]; ;  [2]
  1. Semenov Institute of Chemical Physics, Moscow (Russian Federation)
  2. Russian Medical State Univ., Moscow (Russian Federation)
Publication Date:
OSTI Identifier:
75554
Resource Type:
Journal Article
Journal Name:
Russian Journal of Genetics
Additional Journal Information:
Journal Volume: 31; Journal Issue: 4; Other Information: PBD: Apr 1995; TN: Translated from Genetika; 31: No. 4, 485-491(1995)
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; 56 BIOLOGY AND MEDICINE, APPLIED STUDIES; CHROMOSOMES; CHROMOSOMAL ABERRATIONS; MUTAGENESIS; GENES; BIOLOGICAL RADIATION EFFECTS; DNA; SYNTHESIS; LYMPHOCYTES; NUCLEOTIDES

Citation Formats

Akif`ev, A P, Khudolii, G A, Krasnopevtsev, A V, Yakimenko, A V, and Khandogina, E K. The G{sub 1} process in PHA-stimulated human lymphocytes and the appearance of radiation-induced chromosome aberrations. United States: N. p., 1995. Web.
Akif`ev, A P, Khudolii, G A, Krasnopevtsev, A V, Yakimenko, A V, & Khandogina, E K. The G{sub 1} process in PHA-stimulated human lymphocytes and the appearance of radiation-induced chromosome aberrations. United States.
Akif`ev, A P, Khudolii, G A, Krasnopevtsev, A V, Yakimenko, A V, and Khandogina, E K. Sat . "The G{sub 1} process in PHA-stimulated human lymphocytes and the appearance of radiation-induced chromosome aberrations". United States.
@article{osti_75554,
title = {The G{sub 1} process in PHA-stimulated human lymphocytes and the appearance of radiation-induced chromosome aberrations},
author = {Akif`ev, A P and Khudolii, G A and Krasnopevtsev, A V and Yakimenko, A V and Khandogina, E K},
abstractNote = {Experimental evidence for the existence of local spontaneous DNA synthesis in PHA-stimulated human lymphocytes at the G{sub 1} phase (G{sub 1} process) is presented. This process is associated with the appearance of single-stranded DNA breaks and their subsequent reparation. DNA sequences involved in the G{sub 1} process were characterized in terms of their nucleotide composition and association with the nuclear matrix. It was shown that the G{sub 1} process occurs in chromosome regions in which radiation-induced exchange-type chromosome aberrations arise. The results of the study are regarded as evidence in favor of a molecular version of the primary contact theory of chromosomal mutagenesis. A possible role of G{sub 1} process disturbances in pathogenesis of chromosome instability syndromes in humans is discussed. 18 refs., 3 figs., 2 tabs.},
doi = {},
url = {https://www.osti.gov/biblio/75554}, journal = {Russian Journal of Genetics},
number = 4,
volume = 31,
place = {United States},
year = {1995},
month = {4}
}