Studies on a conformational change of tobacco ribulose diphosphate carboxylase induced by D-ribulose-1,5-diphosphate. [Nicotiana tabacum]
In the presence of D-ribulose diphosphate, crystalline ribulose diphosphate carboxylase from Nicotiana tabacum leaves undergoes a profound change in solubility. The solubility change did not involve a conformational change in molecular volume exceeding 2 percent as measured by sedimentation velocity suggesting no gross change in quaternary structure. However, the change in solubility did involve a tertiary structural change wherein some previously buried tyrosyl and tryptophyl residues became exposed, as indicated by difference spectrophotometry. Although the enzyme molecule has 8 binding sites for ribulose diphosphate, the conformational change is complete after 4 substrate molecules are bound. A cooperative action among the subunits is proposed.
- Research Organization:
- Univ. of California, Los Angeles
- OSTI ID:
- 7355007
- Journal Information:
- Arch. Biochem. Biophys.; (United States), Vol. 161
- Country of Publication:
- United States
- Language:
- English
Similar Records
Photocontrol of the expression of genes encoding chlorophyll a/b binding proteins and small subunit of ribulose-1,5-bisphosphate carboxylase in etiolated seedlings of Lycopersicon esculentum (L. ) and Nicotiana tabacum (L. )
Evidence for effects on the in vivo activity of ribulose-bisphosphate carboxylase/oxygenase during development of Mn toxicity in tobacco. [Nicotiana tabacum L. cv KY14]
Related Subjects
CARBOXYLASE
MOLECULAR STRUCTURE
SOLUBILITY
BIOCHEMISTRY
CHEMICAL BONDS
CONFIGURATION
ENZYMES
METABOLISM
NICOTIANA
PHOSPHATES
RIBULOSE
BIOMASS
CARBOHYDRATES
CHEMISTRY
ENERGY SOURCES
KETONES
LYASES
MONOSACCHARIDES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PENTOSES
PHOSPHORUS COMPOUNDS
PLANTS
RENEWABLE ENERGY SOURCES
SACCHARIDES
550200* - Biochemistry