Radioimmunoassay for mammalian type C viral reverse transcriptase. [/sup 125/I tracer technique]

Journal Article · Sun May 01 00:00:00 EDT 1977 · J. Virol.; (United States)

OSTI ID:7313405

Krakower, J M; Barbacid, M; Aaronson, S A

Radioimmunological techniques were applied to the analysis of reverse transcriptases of mammalian type C RNA viruses. The polymerase of Rauscher mouse leukemia virus was purified by ion exchange and sequential affinity chromatography. Radioimmunoassays that utilized the viral enzyme as a probe detected as little as 1 ng of purified polymerase. No cross-reactivity could be demonstrated between the reverse transcriptase and other known virus-coded proteins. By comparing the immunological reactivity of the purified enzyme with the reactivity of detergent-disrupted virions, Rauscher mouse leukemia virus was shown to contain the antigenic equivalent of 40 molecules of reverse transcriptase. In a homologous competition immunoassay, the Rauscher viral enzyme demonstrated type-specific antigenic determinants, which distinguish it from other mouse type C viral polymerases. In a broadly reactive interspecies immunoassay, the reverse transcriptases of a number of mammalian type C viruses were cross-reactive, indicating their shared antigenic determinants. Various treatments that inhibited or inactivated DNA polymerase activity had little or no effect on the immunological properties of the enzyme. Thus, radioimmunoassays should be useful in the search for type C viral reverse transcriptase as a marker of subviral expression.

• Research Organization: National Cancer Inst., Bethesda, MD
• OSTI ID: 7313405
• Journal Information: J. Virol.; (United States), Vol. 22:2
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ENZYMES
RADIOIMMUNOASSAY
POLYMERASES
VIRUSES
CHROMATOGRAPHY
DNA
IODINE 125
ION EXCHANGE
TRACER TECHNIQUES
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASITES
RADIOASSAY
RADIOISOTOPES
SEPARATION PROCESSES
550601* - Medicine- Unsealed Radionuclides in Diagnostics