Specific radiolabeling of a cell surface receptor for epidermal growth factor. [/sup 125/I]
A photoreactive derivative of epidermal growth factor (EGF) has been used to identify and specifically label a membrane receptor for EGF on mouse 3T3 cells. Photoactivable EGF, labeled with /sup 125/I, was incubated with 3T3 cells and then photolyzed in situ to generate a nitrene capable of reacting with a wide variety of chemical bonds. Analysis of the system by sodium dodecyl sulfate/polyacrylamide gel electrophoresis revealed, besides the band of EGF, only one other major radioactive band, at a position indicating an apparent molecular weight of 190,000. This band was absent when a nonresponsive and nonbinding variant of 3T3 was used. A direct proportionality between binding activity and crosslinked complex formation was demonstrated using a variety of binding conditions. Down regulated cells, in which EGF binding activity was greatly reduced by prolonged incubation with an appropriate concentration of EGF, also had a decrease in covalent complex formation proportional to the decrease in EGF binding activity.
- Research Organization:
- Univ. of California, Los Angeles, CA (United States)
- OSTI ID:
- 7312218
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 74:7
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
LABELLING
EPIDERMIS
POLYPEPTIDES
PHOTOLYSIS
BINDING ENERGY
CELL MEMBRANES
CHEMICAL REACTIONS
ELECTROPHORESIS
GROWTH
IODINE 125
MICE
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ELECTRON CAPTURE RADIOISOTOPES
ENERGY
EPITHELIUM
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
MAMMALS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PHOTOCHEMICAL REACTIONS
PROTEINS
RADIOISOTOPES
RODENTS
SKIN
TISSUES
VERTEBRATES
550200* - Biochemistry