Cloned ribosomal RNA genes from chloroplasts of Euglena gracilis. [Escherichia coli]

Journal Article · Fri Apr 08 00:00:00 EST 1977 · Science; (United States)

OSTI ID:7312201

Lomax, M I ^[1]; Helling, R B; Hecker, L I; Schwartzbach, S D; Barnett, W E

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1. Univ. of Michigan, Ann Arbor

Fragments of Euglena chloroplast DNA generated by endonuclease R . Eco RI were separated by agarose-gel electrophoresis into 24 distinct bands. At least five fragments contain sequences complementary to chloroplast ribosomal RNA. Most of the Eco RI fragments have been cloned in a plasmid of Escherichia coli. Three of the cloned fragments were shown to contain chloroplast ribosomal RNA sequences by DNA-RNA hybridization.

• OSTI ID: 7312201
• Journal Information: Science; (United States), Vol. 196:4286
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHLOROPLASTS
EUGLENA
GENES
RNA
DNA
ELECTROPHORESIS
ESCHERICHIA COLI
HYBRIDIZATION
NUCLEASES
RIBOSOMES
ALGAE
BACTERIA
BIOMASS
CELL CONSTITUENTS
ENERGY SOURCES
ENZYMES
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
PHOSPHOTRANSFERASES
PLANTS
RENEWABLE ENERGY SOURCES
TRANSFERASES
UNICELLULAR ALGAE
550400* - Genetics