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Title: Bacteriophage system for screening and study of biologically active polycyclic aromatic hydrocarbons and related compounds

Abstract

The usefulness of bacterial viruses for detecting substances that are potentially carcinogenic is reexamined as a model system for screening biologically active polycyclic aromatic hydrocarbons. A modification of the original assay procedure allows one to distinguish between aromatics that can modify the biological activity of infectious nucleic acids directly and those polycyclic aromatic hydrocarbons that require metabolic activation by Escherichia coli enzymes. The effect of chemical modification of several different polycyclic aromatic hydrocarbons, with respect to their biological activity in the phage assay system, is described. Among the 31 different compounds examined, (+-)anti-benzo(a)pyrene-7,8-diol-9,10-epoxide was the most potent inhibitor of infectious phage nucleic acid. The (+) and (-) isomers of the above racemic mixture did not differ significantly in their capacity to inhibit phage replication.

Authors:
; ; ;
Publication Date:
Research Org.:
Franklin McLean Memorial Research Inst., Chicago
OSTI Identifier:
7312150
Resource Type:
Journal Article
Journal Name:
Proc. Natl. Acad. Sci. U.S.A.; (United States)
Additional Journal Information:
Journal Volume: 74:4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; POLYCYCLIC AROMATIC HYDROCARBONS; BIOASSAY; BACTERIOPHAGES; CARCINOGENESIS; INHIBITION; METABOLISM; NUCLEIC ACIDS; AROMATICS; HYDROCARBONS; MICROORGANISMS; ORGANIC COMPOUNDS; PARASITES; PATHOGENESIS; VIRUSES; 550700* - Microbiology

Citation Formats

Hsu, W T, Harvey, R G, Lin, E J.S., and Weiss, S B. Bacteriophage system for screening and study of biologically active polycyclic aromatic hydrocarbons and related compounds. United States: N. p., 1977. Web. doi:10.1073/pnas.74.4.1378.
Hsu, W T, Harvey, R G, Lin, E J.S., & Weiss, S B. Bacteriophage system for screening and study of biologically active polycyclic aromatic hydrocarbons and related compounds. United States. doi:10.1073/pnas.74.4.1378.
Hsu, W T, Harvey, R G, Lin, E J.S., and Weiss, S B. Fri . "Bacteriophage system for screening and study of biologically active polycyclic aromatic hydrocarbons and related compounds". United States. doi:10.1073/pnas.74.4.1378.
@article{osti_7312150,
title = {Bacteriophage system for screening and study of biologically active polycyclic aromatic hydrocarbons and related compounds},
author = {Hsu, W T and Harvey, R G and Lin, E J.S. and Weiss, S B},
abstractNote = {The usefulness of bacterial viruses for detecting substances that are potentially carcinogenic is reexamined as a model system for screening biologically active polycyclic aromatic hydrocarbons. A modification of the original assay procedure allows one to distinguish between aromatics that can modify the biological activity of infectious nucleic acids directly and those polycyclic aromatic hydrocarbons that require metabolic activation by Escherichia coli enzymes. The effect of chemical modification of several different polycyclic aromatic hydrocarbons, with respect to their biological activity in the phage assay system, is described. Among the 31 different compounds examined, (+-)anti-benzo(a)pyrene-7,8-diol-9,10-epoxide was the most potent inhibitor of infectious phage nucleic acid. The (+) and (-) isomers of the above racemic mixture did not differ significantly in their capacity to inhibit phage replication.},
doi = {10.1073/pnas.74.4.1378},
journal = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number = ,
volume = 74:4,
place = {United States},
year = {1977},
month = {4}
}