Relationships between hydroxyproline-containing proteins secreted into the cell wall and medium by suspension-cultured Acer psedoplatanus cells
The pathway of hydroxyproline-containing proteins to the cell wall and to the growth medium in suspension-cultured Acer pseudoplatanus cells is traced by following the kinetics of the transfer of protein-bound /sup 14/C-hydroxyproline into various fractions, and by comparing the hydroxyproline-arabinoside profiles of these fractions after alkaline hydrolysis. Hydroxyproline-rich protein passes directly from a membrane-bound compartment in the cytoplasm to the cell wall, not via an intermediate salt-soluble pool in the wall. There are at least three hydroxyproline-containing glycoproteins in the cell wall. One which possesses mono-, tri-, and tetraarabinoside side chains accounts for over 90% of the total hydroxyproline. This glycoprotein is ''extensin.'' The hydroxyproline-containing proteins secreted into the medium have a glycosylation pattern markedly different from that of the major cell wall glycoprotein. It appears that there is little or no wall-like extensin in the medium. Approximately half of the protein-bound hydroxyproline secreted into the medium is linked to an arabinogalactan. This linkage is also found in a particulate wall protein precursor fraction from the cytoplasm, but only trace amounts can be detected in the cell wall.
- Research Organization:
- Michigan State Univ., East Lansing
- OSTI ID:
- 7291163
- Journal Information:
- Plant Physiol.; (United States), Vol. 59:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HYDROXYPROLINE
PLANT CELLS
BIOCHEMICAL REACTION KINETICS
PROTEINS
CARBON 14
CELL WALL
CYTOPLASM
GLUCOPROTEINS
TREES
AMINES
AMINO ACIDS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOMASS
CARBOHYDRATES
CARBON ISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
ENERGY SOURCES
EVEN-EVEN NUCLEI
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
ISOTOPES
KINETICS
LIGHT NUCLEI
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
PYRROLES
PYRROLIDINES
RADIOISOTOPES
REACTION KINETICS
RENEWABLE ENERGY SOURCES
SACCHARIDES
YEARS LIVING RADIOISOTOPES
550300* - Cytology
550200 - Biochemistry